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Literature summary for 2.7.2.1 extracted from

  • Ichikawa, Y.; Mori, N.; Miki, S.; Hosoi, N.; Kitamoto, Y.
    Purification and properties of acetate kinase from propionibacterium freudenreichii (1985), J. Ferment. Technol., 63, 143-149.
No PubMed abstract available
Search for more literature for 2.7.2.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 Propionyl phosphate pH 7.5, 30°C Propionibacterium freudenreichii
0.2
-
 ADP pH 7.5, 30°C Propionibacterium freudenreichii
0.22
-
 ATP pH 7.5, 30°C Propionibacterium freudenreichii
0.44
-
 acetyl phosphate pH 7.5, 30°C Propionibacterium freudenreichii
63.3
-
 propionate pH 7.5, 30°C Propionibacterium freudenreichii
80.9
-
 acetate pH 7.5, 30°C Propionibacterium freudenreichii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Propionibacterium freudenreichii
Co2+
-
 Propionibacterium freudenreichii
Mg2+
-
 Propionibacterium freudenreichii
Mn2+
-
 Propionibacterium freudenreichii
additional information no activity with Cu2+, Ni2+, Hg2+, Ba2+ or Fe2+ Propionibacterium freudenreichii
Zn2+
-
 Propionibacterium freudenreichii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 6 * 41000, SDS-PAGE Propionibacterium freudenreichii
240000
-
 gel filtration Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Propionibacterium freudenreichii
-
 ADP + acetyl phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Propionibacterium freudenreichii
-
 IFO 12424
-

Purification (Commentary)

Purification (Comment) Organism
-
 Propionibacterium freudenreichii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
-
 Propionibacterium freudenreichii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
 Propionibacterium freudenreichii ADP + acetyl phosphate
-
 r
ATP + propionate
-
 Propionibacterium freudenreichii ADP + propionyl phosphate
-
 r
GTP + acetate
-
 Propionibacterium freudenreichii GDP + acetyl phosphate
-
 r
additional information not active toward formate, butyrate, valerate and succinate Propionibacterium freudenreichii ?
-
 ?
UTP + acetate
-
 Propionibacterium freudenreichii UDP + acetyl phosphate
-
 r

Subunits

Subunits Comment Organism
hexamer 6 * 41000, SDS-PAGE Propionibacterium freudenreichii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Propionibacterium freudenreichii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 60 84% remaining activity at 35°C, 49% at 45°C, almost zero at 60°C Propionibacterium freudenreichii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Propionibacterium freudenreichii
7.5
-
-
 Propionibacterium freudenreichii

pH Range

pH Minimum pH Maximum Comment Organism
7 8 most active between Propionibacterium freudenreichii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 stable in this range Propionibacterium freudenreichii