Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-glycerophosphate | - |
Geobacillus stearothermophilus | |
D-fructose 1,6-bisphosphate | activates formation of ADP | Geobacillus stearothermophilus | |
D-Glucose 1,6-bisphosphate | - |
Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Geobacillus stearothermophilus | |
Mn2+ | - |
Geobacillus stearothermophilus | |
additional information | Co2+, Ca2+, Cd2+ and Zn2+ can replace Mg2+ or Mn2+ only partially | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Geobacillus stearothermophilus |
160000 | 170000 | gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | Geobacillus stearothermophilus | function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase | ADP + acetyl phosphate | - |
r | |
ATP + acetate | Geobacillus stearothermophilus NCA 1503 | function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase | ADP + acetyl phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
NCA 1503 | - |
Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1530 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate | - |
Geobacillus stearothermophilus | ADP + acetyl phosphate | - |
r | |
ATP + acetate | function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase | Geobacillus stearothermophilus | ADP + acetyl phosphate | - |
r | |
ATP + acetate | - |
Geobacillus stearothermophilus NCA 1503 | ADP + acetyl phosphate | - |
r | |
ATP + acetate | function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase | Geobacillus stearothermophilus NCA 1503 | ADP + acetyl phosphate | - |
r | |
ATP + propionate | poor acceptor, 5% relative to acetate at 500 mM | Geobacillus stearothermophilus | ADP + propionyl phosphate | - |
r | |
ATP + propionate | poor acceptor, 5% relative to acetate at 500 mM | Geobacillus stearothermophilus NCA 1503 | ADP + propionyl phosphate | - |
r | |
GTP + acetate | - |
Geobacillus stearothermophilus | GDP + acetyl phosphate | - |
r | |
GTP + acetate | - |
Geobacillus stearothermophilus NCA 1503 | GDP + acetyl phosphate | - |
r | |
additional information | UTP and CTP are active only partially | Geobacillus stearothermophilus | ? | - |
? | |
additional information | UTP and CTP are active only partially | Geobacillus stearothermophilus NCA 1503 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
quite heat stable | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | stable, rapid inactivation at pH values lower than 6.0, rather stable at alkaline pH | Geobacillus stearothermophilus |