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Literature summary for 2.7.13.3 extracted from

  • Brooks, J.F.; Mandel, M.J.
    The histidine kinase BinK is a negative regulator of biofilm formation and squid colonization (2016), J. Bacteriol., 198, 2596-2607.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.13.3

Cloned(Commentary)

Cloned (Comment) Organism
gene VF_A0360, genotyping, overview, cloning of plasmid pTn7BinK Aliivibrio fischeri

Protein Variants

Protein Variants Comment Organism
additional information construction of enzyme-deficient mutant strain rscS*DELTAbinK sypG::Tnerm, competition assays of wild-type and mutant strains in vitro and in vivo, overview. DELTAbinK has no effect in the pSypG sypE+ background Aliivibrio fischeri

Organism

Organism UniProt Comment Textmining
Aliivibrio fischeri Q5E0L6
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Aliivibrio fischeri ATCC 700601 Q5E0L6
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Synonyms

Synonyms Comment Organism
BinK
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 Aliivibrio fischeri
sensor histidine kinase
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 Aliivibrio fischeri
VF_A0360
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 Aliivibrio fischeri

General Information

General Information Comment Organism
malfunction binK mutants exhibit a colonization advantage in a global genetic screen, phenotype, overview. Bacterial biofilm aggregates in the host are larger in strains lacking BinK, whereas overexpression of BinK suppresses biofilm formation and squid colonization Aliivibrio fischeri
physiological function gene VF_A0360 encodes the biofilm inhibitor kinase binK, an orphan hybrid histidine kinase that negatively regulates the Vibrio fischeri symbiotic biofilm (Syp) in vivo and in vitro. Colonization of Euprymna scolopes bobtail squid by Vibrio fischeri bacteria requires bacterial aggregation in host mucus as the symbiont transitions from a planktonic lifestyle in seawater to a biofilm-associated state in the host. Enzyme BinK acts upstream of SypG, the sigma54-dependent transcriptional regulator of the syp biofilm locus. The BinK effects are dependent on intact signaling in the RscS-Syp biofilm pathway. Enzyme BinK antagonizes the signal from RscS and serves as an integral component in Vibrio fischeri biofilm regulation. The hybrid histidine kinase BinK is a negative regulator of in vivo aggregation and squid colonization and of Syp biofilm phenotypes in culture. BinK is acting through SypG and not SypE Aliivibrio fischeri