Cloned (Comment) | Organism |
---|---|
cloning of the extracellular sensory domain of DraK, consisting of 88 residues (Glu28-Arg115) from genomic DNA, and overexpression as N-terminally His6-tagged protein in Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
Crystallization (Comment) | Organism |
---|---|
purified recombinant N-terminally His6-tagged extracellular sensory domain of DraK, sitting drop vapour diffusion method, mixing 200 nl of of 12 mg/ml protein in 10 mM sodium acetate, pH 4.5, and 100 mM NaCl with reservoir solution containing 0.2 M potassium/sodium tartrate, and 20% PEG 3350, and equilibration against 0.070 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement | Streptomyces coelicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | transmembrane protein | Streptomyces coelicolor | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9KZ83 | - |
- |
Streptomyces coelicolor ATCC BAA-471D | Q9KZ83 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged extracellular sensory domain of DraK from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration | Streptomyces coelicolor |
Subunits | Comment | Organism |
---|---|---|
More | enzyme DraK consists of an extracellular domain flanked by two transmembrane helices (Leu5-Val27 and Leu126-Ile145), a sensory domain (Glu28-Thr125), a HAMP (histidine kinases, adenylyl cyclases, methyl-accepting chemotaxis proteins and phosphatases) domain (Asn149-Thr201) and a kinase domain (Ala202-Pro410) composed of His KA and CA domains | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
Drak | - |
Streptomyces coelicolor |
DraK histidine kinase | - |
Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
physiological function | DraK is a sensory histidine kinase involved in the differential regulation of antibiotics in Streptomyces coelicolor through the DraR/DraK two-component system | Streptomyces coelicolor |