Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodospirillum centenum |
expression of His10-tagged full-length wild-type Ppr in Escherichia coli strain BL21(DE3), overexpression of the PYP domain of Ppr in Escherichia coli, co-expression of the full-length enzyme with tyrosine ammonia-lyase and p-coumaric acid ligase | Rhodospirillum centenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rhodospirillum centenum | role of the C-terminal domains in the photocycle of a light sensor histidine kinase Ppr having a photoactive yellow protein, PYP, domain as the photosensor domain, photocycles of the PYP domain of Ppr and of the full-length Ppr. The, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum centenum | - |
- |
- |
Rhodospirillum centenum | - |
gene ppr | - |
Purification (Comment) | Organism |
---|---|
Ni-chelating Sepharose column chromatography and Sephacryl S300 gel filtration | Rhodospirillum centenum |
recombinant His10-tagged full-length wild-type Ppr from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rhodospirillum centenum |
Renatured (Comment) | Organism |
---|---|
chemical reconstitution of the recombinantly expressed PYP domain of Ppr with 4-coumaric acid | Rhodospirillum centenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | role of the C-terminal domains in the photocycle of a light sensor histidine kinase Ppr having a photoactive yellow protein, PYP, domain as the photosensor domain, photocycles of the PYP domain of Ppr and of the full-length Ppr. The, overview | Rhodospirillum centenum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Ppr is composed of three domains, a PYP domain, a bacteriophytochrome Bph domain, and a histidine kinase domain, in the order from the N- to the C-terminal of Ppr | Rhodospirillum centenum |
Synonyms | Comment | Organism |
---|---|---|
light sensor histidine kinase | - |
Rhodospirillum centenum |
photoactive yellow protein phytochrome-related protein | - |
Rhodospirillum centenum |
Ppr | - |
Rhodospirillum centenum |
PYP phytochrome-related protein | - |
Rhodospirillum centenum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 8.1 | the light/dark difference spectra of Ppr-PYP are pH sensitive, pH-dependent equilibrium change between Ppr-PYPL and Ppr-PYPM, overview | Rhodospirillum centenum |