Cloned (Comment) | Organism |
---|---|
expression of His-tagged 1-121 and truncated DELTA7 H-NOXA domains in Escherichia coli strain BL21(DE3) | Nostoc punctiforme |
Crystallization (Comment) | Organism |
---|---|
dimerized signal transduction histidine kinase domain H-NOXA domain residues 8-121, 4°C, sitting-drop vapor diffusion, 20 mg/ml NpSTHK8121 in 5 mM Tris, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol, is mixed with an equal volume of reservoir solution containing 1.7-1.9 M ammonium sulfate, 100 mM Tris-HCl, pH 7.7-9.0, for crystallization of the full-length enzyme, 15 mg/ml protein is mixed with equal volume of 0.1 M HEPES, pH 7.5, and 1.5 M lithium sulfate monohydrate, crystals appear after 3 days at 20°C, X-ray diffraction structure determination and analysis at 2.1 A resolution | Nostoc punctiforme |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of 7 N-terminal enzyme residues affects dimer organization | Nostoc punctiforme |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | - |
strain PCC 73102 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged 1-121 and truncated DELTA7 H-NOXA domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Nostoc punctiforme |
Subunits | Comment | Organism |
---|---|---|
dimer | the signal transduction histidine kinase domain, H-NOXA revealing a Per-Arnt-Sim fold, monomers dimerize in a parallel arrangement juxtaposing their N-terminal helices and preceding residues, overview | Nostoc punctiforme |
More | the N-terminal domain in an STHK from Nostoc punctiforme is homologous to the central region in soluble guanylyl cyclase, the main receptor for nitric oxide, structure-function analysis and dimerization mechanism, overview | Nostoc punctiforme |
Synonyms | Comment | Organism |
---|---|---|
signal transduction histidine kinase | - |
Nostoc punctiforme |
StHK | - |
Nostoc punctiforme |