KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.75 | - |
ATP | HK1 | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for HK1 activity | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA affinity column, HK1 purified to homogeneity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + histidine kinase HK2 | - |
Mycobacterium tuberculosis | ADP + histidine kinase HK2 N-phospho-L-histidine | - |
? | |
ATP + histidine kinase HK2 | - |
Mycobacterium tuberculosis H37Rv | ADP + histidine kinase HK2 N-phospho-L-histidine | - |
? | |
additional information | neither HK1 nor HK2 is able to autophosphorylate itself, HK1 is an ATP binding protein, acts as a functional kinase and phosphorylates HK2 by interacting with it, transfer of a phosphoryl group from HK2 to the response regulator TcrA | Mycobacterium tuberculosis | ? | - |
? | |
additional information | neither HK1 nor HK2 is able to autophosphorylate itself, HK1 is an ATP binding protein, acts as a functional kinase and phosphorylates HK2 by interacting with it, transfer of a phosphoryl group from HK2 to the response regulator TcrA | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
histidine kinase | - |
Mycobacterium tuberculosis |
HK1 | - |
Mycobacterium tuberculosis |
HK2 | - |
Mycobacterium tuberculosis |