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Literature summary for 2.7.13.3 extracted from

  • Qin, L.; Cai, S.; Zhu, Y.; Inouye, M.
    Cysteine-scanning analysis of the dimerization domain of EnvZ, an osmosensing histidine kinase (2003), J. Bacteriol., 185, 3429-3435.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.13.3

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes cloned and expressed in Escherichia coli strain BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
D286C t1/2 at room temperature is 16 min, compared to 12 min for wild-type enzyme. 71% of the wild-type phosphatase activity Escherichia coli
E257C t1/2 at room temperature is 30 min, compared to 12 min for wild-type enzyme. 31% of the wild-type phosphatase activity Escherichia coli
E261C t1/2 at room temperature is 15 min, compared to 12 min for wild-type enzyme. 77% of the wild-type phosphatase activity Escherichia coli
E268C t1/2 at room temperature is 23 min, compared to 12 min for wild-type enzyme. 45% of the wild-type phosphatase activity Escherichia coli
E275C t1/2 at room temperature is 28 min, compared to 12 min for wild-type enzyme. 34% of the wild-type phosphatase activity Escherichia coli
E276C t1/2 at room temperature is 68 min, compared to 12 min for wild-type enzyme. 5% of the wild-type phosphatase activity Escherichia coli
E282C t1/2 at room temperature is 15 min, compared to 12 min for wild-type enzyme. 77% of the wild-type phosphatase activity Escherichia coli
G240C t1/2 at room temperature is 10 min, compared to 12 min for wild-type enzyme. 123% of the wild-type phosphatase activity Escherichia coli
G264C t1/2 at room temperature is 12 min, compared to 12 min for wild-type enzyme. As active as wild-type enzyme Escherichia coli
H243K inactive mutant protein Escherichia coli
H243N inactive mutant protein Escherichia coli
H243S inactive mutant protein Escherichia coli
H243V inactive mutant protein Escherichia coli
H243Y inactive mutant protein Escherichia coli
K272C t1/2 at room temperature is 55 min, compared to 12 min for wild-type enzyme. 10% of the wild-type phosphatase activity Escherichia coli
L236C t1/2 at room temperature is 25 min, compared to 12 min for wild-type enzyme. 40% of the wild-type phosphatase activity Escherichia coli
L254C inactive mutant enzyme, t1/2 at room temperature is 90 min, compared to 12 min for wild-type enzyme Escherichia coli
N271C t1/2 at room temperature is 36 min, compared to 12 min for wild-type enzyme. 23% of the wild-type phosphatase activity Escherichia coli
N278C t1/2 at room temperature is 45 min, compared to 12 min for wild-type enzyme. 15% of the wild-type phosphatase activity Escherichia coli
Q262C t1/2 at room temperature is 11 min, compared to 12 min for wild-type enzyme. 111% of the wild-type phosphatase activity Escherichia coli
Q283C t1/2 at room temperature is 13 min, compared to 12 min for wild-type enzyme. 91% of the wild-type phosphatase activity Escherichia coli
R246C t1/2 at room temperature is 62 min, compared to 12 min for wild-type enzyme. 7% of the wild-type phosphatase activity Escherichia coli
S242C t1/2 at room temperature is 39 min, compared to 12 min for wild-type enzyme. 20% of the wild-type phosphatase activity Escherichia coli
S260C t1/2 at room temperature is 13 min, compared to 12 min for wild-type enzyme. 91% of the wild-type phosphatase activity Escherichia coli
S269C t1/2 at room temperature is 20 min, compared to 12 min for wild-type enzyme. 54% of the wild-type phosphatase activity Escherichia coli
T235C t1/2 at room temperature is 14 min, compared to 12 min for wild-type enzyme. 84% of the wild-type phosphatase activity Escherichia coli
T247R inactive mutant protein Escherichia coli
T250C t1/2 at room temperature is 23 min, compared to 12 min for wild-type enzyme. 45% of the wild-type phosphatase activity Escherichia coli
T256C t1/2 at room temperature is 27 min, compared to 12 min for wild-type enzyme. 36% of the wild-type phosphatase activity Escherichia coli
Y265C t1/2 at room temperature is 20 min, compared to 12 min for wild-type enzyme. 54% of the wild-type phosphatase activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Synonyms

Synonyms Comment Organism
histidine kinase EnvZ
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 Escherichia coli