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Literature summary for 2.7.12.2 extracted from

  • Chen, T.; Zhu, H.; Ke, D.; Cai, K.; Wang, C.; Gou, H.; Hong, Z.; Zhang, Z.
    A MAP kinase kinase interacts with SymRK and regulates nodule organogenesis in Lotus japonicus (2012), Plant Cell, 24, 823-838.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.12.2

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of a fusion protein between SIP2 and the N-terminal CFP (SIP2:SCFPN173) in Nicotiana benthamiana epidermis leaves via Agrobacterium tumefaciens–mediated transient transformation. Recombinant expression of polyHis- or GST-tagged wild-type and mutant enzymes in Escherichia coli. Real-time PCR expression analysis Lotus japonicus

Protein Variants

Protein Variants Comment Organism
K120R generation of kinase-negative mutants of both protein kinases SymRK and SIP2 by replacing the essential Lys residue with Arg at the ATP binding site. This Lys-to-Arg substitution (SymRK-PK-KR and SIP2-KR) abolishes the kinase activity completely, but does not affect the interaction between SymRK and SIP2 Lotus japonicus
additional information construction of SIP2 RNA interference (RNAi) knockdown plants. Construction of plasmids that express a fusion protein between SIP2 and the N-terminal CFP (SIP2:SCFPN173). The constructs are co-expressed in Nicotiana benthamiana epidermis leaves via Agrobacterium tumefaciens-mediated transient transformation Lotus japonicus

Inhibitors

Inhibitors Comment Organism Structure
additional information SymRK acts as an inhibitor of SIP2 kinase activity when MPK6 is used as a substrate, suggesting that SymRK may serve as a negative regulator of the SIP2 signaling pathway. SymRK and SIP2 interact with each other but cannot use one another as a substrate for phosphorylation. SymRK can autophosphorylate itself but fails to phosphorylate the kinase-negative SIP2-KR, suggesting that SymRK is neither a potential phosphorylation target nor a kinase source of SIP2 Lotus japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Lotus japonicus 5737
-
plasma membrane
-
 Lotus japonicus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Lotus japonicus

Organism

Organism UniProt Comment Textmining
Lotus japonicus F8THZ4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Lotus japonicus
-
additional information gene SIP2 is constitutively expressed in all Lotus japonicus tissues examined Lotus japonicus
-
root
-
 Lotus japonicus
-
root nodule
-
 Lotus japonicus
-
stem
-
 Lotus japonicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein
-
 Lotus japonicus ADP + phosphorylated casein
-
 ?
ATP + MAP kinase 6 Arabidopsis thaliana MAP kinase MPK6 Lotus japonicus ADP + phosphorylated Map kinase 6
-
 ?

Synonyms

Synonyms Comment Organism
MAP kinase kinase
-
 Lotus japonicus
MAPKK
-
 Lotus japonicus
Sip2
-
 Lotus japonicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Lotus japonicus

Expression

Organism Comment Expression
Lotus japonicus hairy roots expressing SIP2 RNAi have downregulated SIP2 mRNA levels in about 70% of transgenic hairy roots down
Lotus japonicus inoculation with Mesorhizobium loti MAFF303099 does not induce the SIP2 enzyme expression additional information

General Information

General Information Comment Organism
malfunction knockdown expression of SIP2 via RNA interference (RNAi) results in drastic reduction of nodules formed in transgenic hairy roots. In these roots, the expression levels of SIP2 and three marker genes for infection thread and nodule primordium formation are downregulated drastically, while the expression of two other MAPKK genes are not altered Lotus japonicus
additional information the interaction between SymRK and SIP2 is conserved among legumes, interaction analysis of SIP2 and SymRK from different legume species, overview Lotus japonicus
physiological function SIP2 represents a typical plant mitogen-activated protein kinase kinase (MAPKK) and exhibits autophosphorylation and transphosphorylation activities. SymRK may serve as a negative regulator of the SIP2 signaling pathway. SymRK and SIP2 interaqct and are both protein kinases. The kinase domain of SIP2 is responsible for its interaction with SymRK. Essential role of SIP2 in the early symbiosis signaling and nodule organogenesis Lotus japonicus