Cloned (Comment) | Organism |
---|---|
gene Tb927.10.5270, recombinant expression of MKK5 wild-type and mutant in Escherichia coli strain BL21 | Trypanosoma brucei brucei |
gene Tb927.3.4860, recombinant expression of His-tagged MKK1 wild-type and mutants in Escherichia coli strain BL21 | Trypanosoma brucei brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of gene knockout mutant by cloning approximately 500 bp from the genomic regions flanking gene MKK5 (Tb927.10.5270) into plasmid pLEW13 | Trypanosoma brucei brucei |
additional information | construction of gene knockout mutants by cloning approximately 500 bp from the genomic regions flanking gene MKK1 (Tb927.3.4860) into plasmid pLEW13 | Trypanosoma brucei brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Trypanosoma brucei brucei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Trypanosoma brucei brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | Q38B76 | - |
- |
Trypanosoma brucei brucei | Q582P9 | - |
- |
Trypanosoma brucei brucei 927/4 | Q38B76 | - |
- |
Trypanosoma brucei brucei 927/4 | Q582P9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged MKK1 wild-type and mutants solubilized from inclusion bodies expressed in Eschrichia coli strain BL21 by nickel affinity chromatography | Trypanosoma brucei brucei |
recombinant His-tagged MKK5 wild-type and mutant solubilized from inclusion bodies expressed in Escherichia coli strain BL21 by nickel affinity chromatography | Trypanosoma brucei brucei |
Renatured (Comment) | Organism |
---|---|
recombinant His-tagged MKK1 wild-type and mutants from Escherichia coli strain BL21 inclusion bodies by 8 M urea | Trypanosoma brucei brucei |
recombinant His-tagged MKK5 wild-type and mutant from Escherichia coli strain BL21 inclusion bodies by 8 M urea | Trypanosoma brucei brucei |
Synonyms | Comment | Organism |
---|---|---|
mitogen activated protein kinase kinase | - |
Trypanosoma brucei brucei |
MKK1 | - |
Trypanosoma brucei brucei |
MKK5 | - |
Trypanosoma brucei brucei |
Tb927.10.5270 | - |
Trypanosoma brucei brucei |
Tb927.3.4860 | - |
Trypanosoma brucei brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma brucei brucei |
General Information | Comment | Organism |
---|---|---|
evolution | the protozoan pathogen, Trypanosoma brucei brucei, possesses many protein kinases which show high homology to the catalytic domains of mitogen activated protein kinases (MAPKs) and their upstream regulators. The close relationship of MKK and MKKK kinase domains combined with the phylogenetic distance between trypanosomatids and model organisms makes direct experimentation required to delineate functional attributes of the parasite kinases | Trypanosoma brucei brucei |
malfunction | bloodstream forms lacking MKK1 show decreased growth at 39°C as compared to the parental line, enzyme knockout phenotype, overview. The two mkk1 knockout clones are moderately more sensitive to temperature stress than the wild-type | Trypanosoma brucei brucei |
malfunction | enzyme knockout phenotype, overview | Trypanosoma brucei brucei |
physiological function | mitogen activated kinase kinase gene MKK1 is not essential in the pathogenic bloodstream stage of Trypanosoma brucei, no effcts on growth and virulence either in vitro or in vivo. Trypanosoma brucei MKK1 does not appear to play a significant role in flagellar biogenesis | Trypanosoma brucei brucei |
physiological function | mitogen activated kinase kinase gene MKK5 is not essential in the pathogenic bloodstream stage of Trypanosoma brucei, no effcts on growth and virulence either in vitro or in vivo | Trypanosoma brucei brucei |