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Literature summary for 2.7.12.2 extracted from

  • Jensen, B.C.; Kifer, C.T.; Parsons, M.
    Trypanosoma brucei: two mitogen activated protein kinase kinases are dispensable for growth and virulence of the bloodstream form (2011), Exp. Parasitol., 128, 250-255.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Tb927.10.5270, recombinant expression of MKK5 wild-type and mutant in Escherichia coli strain BL21 Trypanosoma brucei brucei
gene Tb927.3.4860, recombinant expression of His-tagged MKK1 wild-type and mutants in Escherichia coli strain BL21 Trypanosoma brucei brucei

Protein Variants

Protein Variants Comment Organism
additional information construction of gene knockout mutant by cloning approximately 500 bp from the genomic regions flanking gene MKK5 (Tb927.10.5270) into plasmid pLEW13 Trypanosoma brucei brucei
additional information construction of gene knockout mutants by cloning approximately 500 bp from the genomic regions flanking gene MKK1 (Tb927.3.4860) into plasmid pLEW13 Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Trypanosoma brucei brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma brucei brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei Q38B76
-
-
Trypanosoma brucei brucei Q582P9
-
-
Trypanosoma brucei brucei 927/4 Q38B76
-
-
Trypanosoma brucei brucei 927/4 Q582P9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged MKK1 wild-type and mutants solubilized from inclusion bodies expressed in Eschrichia coli strain BL21 by nickel affinity chromatography Trypanosoma brucei brucei
recombinant His-tagged MKK5 wild-type and mutant solubilized from inclusion bodies expressed in Escherichia coli strain BL21 by nickel affinity chromatography Trypanosoma brucei brucei

Renatured (Commentary)

Renatured (Comment) Organism
recombinant His-tagged MKK1 wild-type and mutants from Escherichia coli strain BL21 inclusion bodies by 8 M urea Trypanosoma brucei brucei
recombinant His-tagged MKK5 wild-type and mutant from Escherichia coli strain BL21 inclusion bodies by 8 M urea Trypanosoma brucei brucei

Synonyms

Synonyms Comment Organism
mitogen activated protein kinase kinase
-
Trypanosoma brucei brucei
MKK1
-
Trypanosoma brucei brucei
MKK5
-
Trypanosoma brucei brucei
Tb927.10.5270
-
Trypanosoma brucei brucei
Tb927.3.4860
-
Trypanosoma brucei brucei

Cofactor

Cofactor Comment Organism Structure
ATP
-
Trypanosoma brucei brucei

General Information

General Information Comment Organism
evolution the protozoan pathogen, Trypanosoma brucei brucei, possesses many protein kinases which show high homology to the catalytic domains of mitogen activated protein kinases (MAPKs) and their upstream regulators. The close relationship of MKK and MKKK kinase domains combined with the phylogenetic distance between trypanosomatids and model organisms makes direct experimentation required to delineate functional attributes of the parasite kinases Trypanosoma brucei brucei
malfunction bloodstream forms lacking MKK1 show decreased growth at 39°C as compared to the parental line, enzyme knockout phenotype, overview. The two mkk1 knockout clones are moderately more sensitive to temperature stress than the wild-type Trypanosoma brucei brucei
malfunction enzyme knockout phenotype, overview Trypanosoma brucei brucei
physiological function mitogen activated kinase kinase gene MKK1 is not essential in the pathogenic bloodstream stage of Trypanosoma brucei, no effcts on growth and virulence either in vitro or in vivo. Trypanosoma brucei MKK1 does not appear to play a significant role in flagellar biogenesis Trypanosoma brucei brucei
physiological function mitogen activated kinase kinase gene MKK5 is not essential in the pathogenic bloodstream stage of Trypanosoma brucei, no effcts on growth and virulence either in vitro or in vivo Trypanosoma brucei brucei