Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain DH5alpha as GST-fusion protein, expression in 293A cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knock-out leads to increased D-glucose uptake in response to insulin | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
genistein | - |
Homo sapiens | |
Zn2+ | inhibits the autophosphoylation, which cannot be reversed by Mn2+ addition | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for kinase activity | Homo sapiens | |
Mn2+ | essential for autophosphorylation activity, cannot be substituted by Mg2+, Ca2+, or Zn2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + insulin receptor kinase substrate 1 | Homo sapiens | phosphorylation at serine residues, overview, the enzyme is involved in the insulin signaling pathway | ADP + phosphorylated insulin receptor kinase substrate 1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme autophosphorylates at Thr202, Tyr72 and Tyr83, the insulin-activated insulin receptor kinase phosphorylates Y198 in the YMKM motif, Y228 in the YLSF motif, and Y291 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion protein from Escherichia coli strain DH5alpha by glutathione affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | - |
Homo sapiens | ADP + phosphorylated casein | - |
? | |
ATP + insulin receptor kinase substrate 1 | phosphorylation at serine residues, overview, the enzyme is involved in the insulin signaling pathway | Homo sapiens | ADP + phosphorylated insulin receptor kinase substrate 1 | - |
? | |
ATP + insulin receptor kinase substrate 1 | phosphorylation at serine residues leading to blockage of insulin action, overview | Homo sapiens | ADP + phosphorylated insulin receptor kinase substrate 1 | - |
? | |
ATP + myelin basic protein | - |
Homo sapiens | ADP + phosphorylated myelin basic protein | - |
? | |
additional information | the enzyme performs autophosphorylation at Thr202, Tyr72 and Tyr83, the enzyme also shows biliverdin reductase activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
biliverdin reductase | - |
Homo sapiens |
BVR | - |
Homo sapiens |
More | the enzyme belongs to the insulin receptor substrate family | Homo sapiens |
serine/threonine/tyrosine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |