Activating Compound | Comment | Organism | Structure |
---|---|---|---|
F-actin | i.e. filamentous actin, from rabbit muscle, about 40fold activation, coiled-coil domain binding structure and mechanism, overview, the enzyme's coiled-coil structure mediates its oligomerization, cellular localization, and actin-binding activity of the MHCK A | Dictyostelium discoideum |
Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged isozyme MHCK A in a Dictyostelium discoideum strain lacking endogenous MHCK A, expression of a truncation mutant DELTAcoil-MHCK A comprising residues 1-498 in Escherichia coli as GST-fused protein | Dictyostelium discoideum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the truncation mutant DELTAcoil-MHCK A comprising residues 1-498 is not affected in its autophosphorylation rate by F-actin | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | F-actin binding kinetics | Dictyostelium discoideum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme's coiled-coil structure mediates its oligomerization, cellular localization, and actin-binding activity of the MHCK A | Dictyostelium discoideum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Dictyostelium discoideum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin-heavy-chain] | Dictyostelium discoideum | - |
ADP + [myosin-heavy-chain]phosphate | - |
? | |
additional information | Dictyostelium discoideum | C-terminal phosphorylation of myosin heavy chain is required for regulation of myosin II filament assembly, regulation mechanism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | P42527 | isozyme MHCK A | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged full-length isozyme MHCK A from Dictyostelium discoideum by affinity chromatography, recombinant GST-fusion truncation mutant DELTAcoil-MHCK A from Escherichia coli by affinity chromatography, the GST-tag is cleaved off by thrombin treatment | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin-heavy-chain] | - |
Dictyostelium discoideum | ADP + [myosin-heavy-chain]phosphate | - |
? | |
additional information | C-terminal phosphorylation of myosin heavy chain is required for regulation of myosin II filament assembly, regulation mechanism | Dictyostelium discoideum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme's coiled-coil structure mediates its oligomerization, cellular localization, and actin-binding activity of the MHCK A | Dictyostelium discoideum |
Synonyms | Comment | Organism |
---|---|---|
MHCK A | - |
Dictyostelium discoideum |
myosin heavy chain kinase A | - |
Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
actin binding assay at | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
actin binding assay at | Dictyostelium discoideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Dictyostelium discoideum |