Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylserine | - |
Acanthamoeba castellanii | |
Rac | reverses autoinhibition of the enzyme | Acanthamoeba castellanii |
Cloned (Comment) | Organism |
---|---|
construction of His-tagged or FLAG-tagged N-terminal enzyme peptides by site directed mutagenesis for introduction of start and stop codons, expression in Escherichia coli BL21(DE3) | Acanthamoeba castellanii |
subcloning and expression in Escherichia coli, expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system | Acanthamoeba castellanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+/calmodulin | calmodulin-binding site: amino acid residues 51-80; competes with phospholipids | Acanthamoeba castellanii | |
EGTA | - |
Acanthamoeba castellanii | |
additional information | autoinhibitory domains, overview, autoinhibition is reversed by Rac | Acanthamoeba castellanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly activates unphosphorlyated enzyme | Acanthamoeba castellanii | |
Mg2+ | - |
Acanthamoeba castellanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin I heavy chain | Acanthamoeba castellanii | - |
ADP + myosin I heavy chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba castellanii | Q93107 | purified myosin I heavy chain kinase | - |
Purification (Comment) | Organism |
---|---|
recombinant peptides of the enzymes N-terminus from Escherichia coli, His- or FLAG-tagged, and recombinant enzyme from Sf9 insect cells | Acanthamoeba castellanii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Acanthamoeba castellanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GRGRSSVYS | synthetic peptide with a sequence corresponding to the phosphorylation site of myosin IC | Acanthamoeba castellanii | ADP + GRGRSS(-phosphate)VYS | - |
? | |
ATP + myosin I heavy chain | - |
Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrate is heavy chain of myosin IC | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MIHCK | - |
Acanthamoeba castellanii |
More | member of the p21-activated kinase family | Acanthamoeba castellanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Acanthamoeba castellanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acanthamoeba castellanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Acanthamoeba castellanii |