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Literature summary for 2.7.11.7 extracted from
- Calmodulin-binding and autoinhibitory domains of Acanthamoeba myosin I heavy chain kinase, a p21-activated kinase (PAK) (2001), J. Biol. Chem., 276, 47468-47473.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylserine | - |
Acanthamoeba castellanii |  |
| Rac | reverses autoinhibition of the enzyme | Acanthamoeba castellanii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction of His-tagged or FLAG-tagged N-terminal enzyme peptides by site directed mutagenesis for introduction of start and stop codons, expression in Escherichia coli BL21(DE3) | Acanthamoeba castellanii |
| subcloning and expression in Escherichia coli, expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system | Acanthamoeba castellanii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+/calmodulin | calmodulin-binding site: amino acid residues 51-80; competes with phospholipids | Acanthamoeba castellanii | |
| EGTA | - |
Acanthamoeba castellanii | |
| additional information | autoinhibitory domains, overview, autoinhibition is reversed by Rac | Acanthamoeba castellanii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slightly activates unphosphorlyated enzyme | Acanthamoeba castellanii | |
| Mg2+ | - |
Acanthamoeba castellanii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin I heavy chain | Acanthamoeba castellanii | - |
ADP + myosin I heavy chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthamoeba castellanii | Q93107 | purified myosin I heavy chain kinase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant peptides of the enzymes N-terminus from Escherichia coli, His- or FLAG-tagged, and recombinant enzyme from Sf9 insect cells | Acanthamoeba castellanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Acanthamoeba castellanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + GRGRSSVYS | synthetic peptide with a sequence corresponding to the phosphorylation site of myosin IC | Acanthamoeba castellanii | ADP + GRGRSS(-phosphate)VYS | - |
? | |
| ATP + myosin I heavy chain | - |
Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin I heavy chain | substrate is heavy chain of myosin IC | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MIHCK | - |
Acanthamoeba castellanii |
| More | member of the p21-activated kinase family | Acanthamoeba castellanii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Acanthamoeba castellanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Acanthamoeba castellanii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Acanthamoeba castellanii | |
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