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Literature summary for 2.7.11.7 extracted from

  • Rieker, J.P.; Swanljung-Collins, H.; Montibeller, J.; Collins, J.H.
    Purification and characterization of a calmodulin-dependent myosin heavy chain kinase from intestinal brush border (1987), J. Biol. Chem., 262, 15262-15268.
    View publication on PubMed
Search for more literature for 2.7.11.7

General Stability

General Stability Organism
freeze-thawing inactivates Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
EGTA
-
 Gallus gallus
NaCl above 0.1 M Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement, Ca2+/calmodulin-dependent isozyme Gallus gallus
Mg2+ requirement Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 10 * 50000, SDS-PAGE, asymmetric complex with an axial ratio calculated for prolate ellipsoid of 6.1 Gallus gallus
490000
-
 gel filtration, sucrose density gradient centrifugation Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin heavy chain Gallus gallus
-
 ADP + myosin heavy chain phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 the Ca2+/calmodulin dependent heavy chain kinase has several properties in common with the family of type II calmodulin-dependent protein kinases
-
Gallus gallus
-
 two myosin heavy-chain kinases: a Ca2+/calmodulin dependent and a Ca2+/calmodulin independent enzyme
-

Purification (Commentary)

Purification (Comment) Organism
Ca2+/calmodulin-dependent heavy chain kinase, 84fold Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
epithelium of the intestine, brush border cells Gallus gallus
-
small intestine epithelium Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00063
-
 purified enzyme Gallus gallus

Storage Stability

Storage Stability Organism
0°C, at least 1 month Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + chicken gizzard myosin light chain no activity Gallus gallus ADP + chicken gizzard myosin light chain phosphate
-
 ?
ATP + myosin heavy chain
-
 Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy chain substrate specificity Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy chain phosphorylates threonine residues Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy chain rabbit skeletal myosin heavy chains are phosphorylated Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy chain 0.7 mol phosphate per mol of heavy chain Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy chain best substrate: chicken intestine brush border myosin Gallus gallus ADP + myosin heavy chain phosphate
-
 ?
ATP + myosin heavy chain specific for myosin heavy chains, no phosphorylation of light chains Gallus gallus ADP + myosin heavy chain phosphate
-
 ?

Subunits

Subunits Comment Organism
decamer 10 * 50000, SDS-PAGE, asymmetric complex with an axial ratio calculated for prolate ellipsoid of 6.1 Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about half-maximal activity at pH 6.0, about 90% of maximal activity at pH 7.0 and pH 9.0 Gallus gallus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Gallus gallus