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Literature summary for 2.7.11.7 extracted from

  • Brzeska, H.; Lynch, T.J.; Martin, B.; Corigliano-Murphy, A.; Korn, E.D.
    Substrate specificity of Acanthamoeba myosin I heavy chain kinase as determined with synthetic peptides (1990), J. Biol. Chem., 265, 16138-16144.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylinositol
-
Acanthamoeba castellanii
phosphatidylserine stimulation only of the autophosphorylated enzyme Acanthamoeba castellanii
Phospholipid vesicles autophosphorylation activity is increased 5-10fold in a Ca2+-independent manner Acanthamoeba castellanii
Phospholipid vesicles composed of phosphatidylserine or phosphatidylinositol, not phosphatidylcholine Acanthamoeba castellanii
Phospholipid vesicles composed of phosphatidylethanolamine Acanthamoeba castellanii

Inhibitors

Inhibitors Comment Organism Structure
Myosin I inhibits autophosphorylation Acanthamoeba castellanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km values for derivatives of peptide GRGRSSVYS Acanthamoeba castellanii
0.05
-
GRGRSSVYS pH 7.0, 30°C Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin I heavy chain Acanthamoeba castellanii
-
ADP + myosin I heavy chain phosphate
-
?

Organism

Organism UniProt Comment Textmining
Acanthamoeba castellanii
-
myosin I heavy chain kinase
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Acanthamoeba castellanii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GRGRSSVYS synthetic peptide with a sequence corresponding to the phosphorylation site of myosin IC Acanthamoeba castellanii ADP + GRGRSS(-phosphate)VYS
-
?
ATP + myosin I heavy chain
-
Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain substrate: myosin IB, phosphorylation site is Ser315 Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain substrate myosin IA: phosphorylation of a single threonine Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain substrate is heavy chain of myosin IC Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain myosin I from intestinal brush border Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain substrate: myosin IC, phosphorylation site is Ser311 Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain a basic amino acid is essential on amino-terminal side of phosphorylation site, two are preferable, and a Tyr-residue is essential two residues away on the COOH-terminal side Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
ATP + myosin I heavy chain substrates are heavy chains of myosin IA and IB Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
?
ATP + synthetic peptides variations in length, and number, location and kind of basic residues of the basic sequence of peptide GRGRSSVYS, overview Acanthamoeba castellanii ?
-
?
additional information substrate specificity study Acanthamoeba castellanii ?
-
?
additional information the Mg2+-ATPase activity of the substrate myosin I is increased by its phosphorylation and the binding of F-actin Acanthamoeba castellanii ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Acanthamoeba castellanii

Cofactor

Cofactor Comment Organism Structure
ATP
-
Acanthamoeba castellanii