Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylinositol | - |
Acanthamoeba castellanii | |
phosphatidylserine | stimulation only of the autophosphorylated enzyme | Acanthamoeba castellanii | |
Phospholipid vesicles | autophosphorylation activity is increased 5-10fold in a Ca2+-independent manner | Acanthamoeba castellanii | |
Phospholipid vesicles | composed of phosphatidylserine or phosphatidylinositol, not phosphatidylcholine | Acanthamoeba castellanii | |
Phospholipid vesicles | composed of phosphatidylethanolamine | Acanthamoeba castellanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Myosin I | inhibits autophosphorylation | Acanthamoeba castellanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values for derivatives of peptide GRGRSSVYS | Acanthamoeba castellanii | |
0.05 | - |
GRGRSSVYS | pH 7.0, 30°C | Acanthamoeba castellanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin I heavy chain | Acanthamoeba castellanii | - |
ADP + myosin I heavy chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba castellanii | - |
myosin I heavy chain kinase | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Acanthamoeba castellanii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GRGRSSVYS | synthetic peptide with a sequence corresponding to the phosphorylation site of myosin IC | Acanthamoeba castellanii | ADP + GRGRSS(-phosphate)VYS | - |
? | |
ATP + myosin I heavy chain | - |
Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrate: myosin IB, phosphorylation site is Ser315 | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrate myosin IA: phosphorylation of a single threonine | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrate is heavy chain of myosin IC | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | myosin I from intestinal brush border | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrate: myosin IC, phosphorylation site is Ser311 | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | a basic amino acid is essential on amino-terminal side of phosphorylation site, two are preferable, and a Tyr-residue is essential two residues away on the COOH-terminal side | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + myosin I heavy chain | substrates are heavy chains of myosin IA and IB | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
ATP + synthetic peptides | variations in length, and number, location and kind of basic residues of the basic sequence of peptide GRGRSSVYS, overview | Acanthamoeba castellanii | ? | - |
? | |
additional information | substrate specificity study | Acanthamoeba castellanii | ? | - |
? | |
additional information | the Mg2+-ATPase activity of the substrate myosin I is increased by its phosphorylation and the binding of F-actin | Acanthamoeba castellanii | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acanthamoeba castellanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Acanthamoeba castellanii |