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Literature summary for 2.7.11.7 extracted from
- Purification and characterization of a myosin I heavy chain kinase from Acanthamoeba castellanii (1983), J. Biol. Chem., 258, 10168-10175.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by calmodulin, cAMP | Acanthamoeba castellanii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+/calmodulin | no inhibition | Acanthamoeba castellanii | |
| additional information | strong inhibition of phosphorylation activity by increasing ionic strength | Acanthamoeba castellanii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
ATP | pH 7.5, 30°C | Acanthamoeba castellanii |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Acanthamoeba castellanii | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | not required | Acanthamoeba castellanii | |
| Mg2+ | requirement | Acanthamoeba castellanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 107000 | - |
gel filtration | Acanthamoeba castellanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin I heavy chain | Acanthamoeba castellanii | - |
ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin II heavy chain | Acanthamoeba castellanii | - |
ADP + myosin II heavy chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acanthamoeba castellanii | - |
myosin I heavy chain kinase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography using histone-resin | Acanthamoeba castellanii |
| to near homogeneity | Acanthamoeba castellanii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Acanthamoeba castellanii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.03 | - |
purified enzyme, substrate histone 2A, 0.067 mM | Acanthamoeba castellanii |
| 4.6 | - |
purified enzyme, substrate myosin IB, 0.0024 mM | Acanthamoeba castellanii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 50% glycerol, 3 months stable and less than 20% loss of activity within 6 months | Acanthamoeba castellanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + casein | poor substrate | Acanthamoeba castellanii | ADP + casein phosphate | - |
? | |
| ATP + histone 2A | - |
Acanthamoeba castellanii | ADP + histone 2A phosphate | - |
? | |
| ATP + myosin heavy chain kinase | intramolecular autophosphorylation | Acanthamoeba castellanii | ADP + myosin heavy chain kinase phosphate | - |
? | |
| ATP + myosin I heavy chain | - |
Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin I heavy chain | substrate: myosin IB, phosphorylation site is Ser315 | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin I heavy chain | phosphorylates serine-residues | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin I heavy chain | incorporation of 0.9-1.0 mol phosphate per mol of heavy chain myosin | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin I heavy chain | contains two myosin heavy chain kinases: one for myosin I and one for myosin II | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin I heavy chain | substrates are heavy chains of myosin IA and IB | Acanthamoeba castellanii | ADP + myosin I heavy chain phosphate | - |
? | |
| ATP + myosin II heavy chain | - |
Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
| ATP + myosin II heavy chain | contains two myosin heavy chain kinases: one for myosin I and one for myosin II | Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
| ATP + myosin II heavy chain | phosphorylates serine residues | Acanthamoeba castellanii | ADP + myosin II heavy chain phosphate | - |
? | |
| ATP + phosvitin | no activity | Acanthamoeba castellanii | ADP + phosvitin phosphate | - |
? | |
| ATP + smooth muscle myosin light chain | - |
Acanthamoeba castellanii | ADP + smooth muscle myosin light chain phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 107000, phosphorylated enzyme, SDS-PAGE | Acanthamoeba castellanii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Acanthamoeba castellanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Acanthamoeba castellanii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | about half-maximal activity at pH 6.0 | Acanthamoeba castellanii |
| 6 | 8.5 | about 80% activity at pH 6.0 | Acanthamoeba castellanii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Acanthamoeba castellanii | |
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