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Literature summary for 2.7.11.7 extracted from

  • Lynch, T.J.; Brzeska, H.; Baines, I.C.; Korn, E.D.
    Purification of myosin I and myosin I heavy chain kinase from Acanthamoeba castellanii (1991), Methods Enzymol., 196, 12-23.
    View publication on PubMed
Search for more literature for 2.7.11.7

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is stimulated by its autophosphorylation Acanthamoeba castellanii
phosphatidylserine stimulates the enzymes autophosphorylation activity Acanthamoeba castellanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 GRGRSSVYS pH 7.5, 30°C Acanthamoeba castellanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
97000
-
 1 * 97000, unphosphorylated enzyme, SDS-PAGE Acanthamoeba castellanii
107000
-
 1 * 107000, phosphorylated enzyme, SDS-PAGE Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin I heavy chain Acanthamoeba castellanii
-
 ADP + myosin I heavy chain phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Acanthamoeba castellanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography using histone-resin Acanthamoeba castellanii

Reaction

Reaction Comment Organism Reaction ID
ATP + [myosin heavy-chain] = ADP + [myosin heavy-chain] phosphate activation of the enzyme by autophosphorylation is necessary for full catalytic activity Acanthamoeba castellanii
a

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Acanthamoeba castellanii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
-
 Acanthamoeba castellanii

Storage Stability

Storage Stability Organism
-20°C, purified, stable up to 1 year Acanthamoeba castellanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GRGRSSVYS synthetic peptide with a sequence corresponding to the phosphorylation site of myosin IC Acanthamoeba castellanii ADP + GRGRSS(-phosphate)VYS
-
 ?
ATP + myosin heavy chain kinase intramolecular autophosphorylation Acanthamoeba castellanii ADP + myosin heavy chain kinase phosphate
-
 ?
ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Acanthamoeba castellanii ADP + myosin heavy chain kinase phosphate
-
 ?
ATP + myosin heavy chain kinase enzyme incorporates 6-8 mol phosphate per mol of enzyme Acanthamoeba castellanii ADP + myosin heavy chain kinase phosphate
-
 ?
ATP + myosin I heavy chain
-
 Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain phosphorylates threonine residues Acanthamoeba castellanii ADP + myosin I heavy chain phosphate
-
 ?
additional information the Mg2+-ATPase activity of the substrate myosin I is increased by its phosphorylation and the binding of F-actin Acanthamoeba castellanii ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 97000, unphosphorylated enzyme, SDS-PAGE Acanthamoeba castellanii
monomer 1 * 107000, phosphorylated enzyme, SDS-PAGE Acanthamoeba castellanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Acanthamoeba castellanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Acanthamoeba castellanii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Acanthamoeba castellanii