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Literature summary for 2.7.11.7 extracted from

  • Ravid, S.; Spudich, J.A.
    Myosin heavy chain kinase from developed Dictyostelium cells. Purification and characterization (1989), J. Biol. Chem., 264, 15144-15150.
    View publication on PubMed
Search for more literature for 2.7.11.7

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by cGMP Dictyostelium discoideum
additional information no activation by calmodulin, cAMP Dictyostelium discoideum

Inhibitors

Inhibitors Comment Organism Structure
KCl 80% inhibition at 90 mM Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 [myosin heavy-chain] pH 7.5, 22°C Dictyostelium discoideum
0.015
-
 [protein LMM58 heavy chain] pH 7.5, 22°C Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Dictyostelium discoideum 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ not required Dictyostelium discoideum
Mg2+ requirement Dictyostelium discoideum
Mg2+ maximal activity at 6-8 mM Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 x * 84000, SDS-PAGE Dictyostelium discoideum
94000
-
 x * 94000, phosphorylated enzyme, SDS-PAGE Dictyostelium discoideum
240000
-
 gel filtration Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin I heavy chain Dictyostelium discoideum involved in regulation of myosin II filament assembly ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain Dictyostelium discoideum increased activity during chemotaxis ADP + myosin I heavy chain phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
 two heavy chain kinases: one in growth-phase cells and one in starved, developing cells
-

Purification (Commentary)

Purification (Comment) Organism
solubilized by high-salt extraction, affinity chromatography, 4600fold, to homogeneity Dictyostelium discoideum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture starved, developing cells Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
 purified enzyme Dictyostelium discoideum

Storage Stability

Storage Stability Organism
-20°C, in 25 mM HEPES buffer, pH 7.5, 1 mM DTT, 1 mM EDTA, 50 mM NaCl, 10% glycerol, or 20% sucrose, stable for 3 months Dictyostelium discoideum
-20°C, in 50% sucrose or glycerol, t1/2: 2 weeks Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein no activity Dictyostelium discoideum ADP + casein phosphate
-
 ?
ATP + myosin heavy chain kinase intramolecular autophosphorylation Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
ATP + myosin heavy chain kinase 4 phosphorylation sites on each heavy chain Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
ATP + myosin heavy chain kinase leads to activation of the phosphorylation activity towards myosin I Dictyostelium discoideum ADP + myosin heavy chain kinase phosphate
-
 ?
ATP + myosin I heavy chain myosin light chains are no substrates Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain 4 mol phosphate per mol of myosin Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain phosphorylates threonine residues Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain 20 mol phosphate per mol of kinase subunit, kinetics Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain bovine muscle myosin is no substrate Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain involved in regulation of myosin II filament assembly Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + myosin I heavy chain increased activity during chemotaxis Dictyostelium discoideum ADP + myosin I heavy chain phosphate
-
 ?
ATP + peptide LMM58 of heavy chain 4 mol phosphate per mol, Thr-residues are phosphorylated Dictyostelium discoideum ?
-
 ?
ATP + [myosin heavy-chain]
-
 Dictyostelium discoideum ADP + [myosin heavy-chain] phosphate
-
 ?
ATP + [protein LMM58 heavy chain]
-
 Dictyostelium discoideum ADP + [protein LMM58 heavy chain] phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 84000, SDS-PAGE Dictyostelium discoideum
? x * 94000, phosphorylated enzyme, SDS-PAGE Dictyostelium discoideum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Dictyostelium discoideum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Dictyostelium discoideum