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Literature summary for 2.7.11.5 extracted from

  • Nimmo, G.A.; Nimmo, H.G.
    The regulatory properties of isocitrate dehydrogenase kinase and isocitrate dehydrogenase phosphatase from Escherichia coli ML308 and the roles of these activities in the control of isocitrate dehydrogenase (1984), Eur. J. Biochem., 141, 409-414.
    View publication on PubMed
Search for more literature for 2.7.11.5

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate inhibits kinase activity Escherichia coli
ADP kinase hyperbolically inhibited Escherichia coli
AMP kinase hyperbolically inhibited Escherichia coli
DL-isocitrate inhibits only kinase activity Escherichia coli
glyoxylate
-
 Escherichia coli
NADPH
-
 Escherichia coli
oxaloacetate inhibits kinase activity Escherichia coli
phosphoenolpyruvate kinase hyperbolically inhibited Escherichia coli
pyruvate inhibits kinase activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 [isocitrate dehydrogenase (NADP+)] pH 7.3, 37°C, kinase activity Escherichia coli
0.088
-
 ATP pH 7.3, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement, isocitrate dehydrogenase phosphatase responds hyperbolically to Mg2+ ions Escherichia coli
additional information Mn2+ or Ca2+ cannot replace Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [isocitrate dehydrogenase (NADP+)] Escherichia coli phosphorylation of isocitrate dehydrogenase during growth on acetate is to render this enzyme rate-limiting in the citric acid cycle, this should cause an increase in the level of isocitrate and divert the flux of carbon through the glyoxylate bypass ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 ML308, ATCC 15224
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [isocitrate dehydrogenase (NADP+)]
-
 Escherichia coli ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 r
ATP + [isocitrate dehydrogenase (NADP+)] phosphorylation of isocitrate dehydrogenase during growth on acetate is to render this enzyme rate-limiting in the citric acid cycle, this should cause an increase in the level of isocitrate and divert the flux of carbon through the glyoxylate bypass Escherichia coli ADP + [isocitrate dehydrogenase (NADP+)] phosphate
-
 r
additional information uses only ATP, no other nucleoside triphospates as only very poor phosphate donors for the kinase activity, GTP and UTP can activate the phosphatase activity to some extent Escherichia coli ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 kinase activity Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ADP isocitrate dehydrogenase phosphatase requires a nucleotide for activity Escherichia coli
ATP isocitrate dehydrogenase phosphatase requires a nucleotide for activity Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.023
-
 DL-isocitrate pH 7.3, 37°C Escherichia coli
0.042
-
 NADPH pH 7.3, 37°C Escherichia coli
0.056
-
 AMP pH 7.3, 37°C Escherichia coli
0.45
-
 ADP pH 7.3, 37°C Escherichia coli
0.55
-
 phosphoenolpyruvate pH 7.3, 37°C Escherichia coli