Cloned (Comment) | Organism |
---|---|
fusion protein with maltose-binding protein | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | Rattus norvegicus | phosphorylation inactivates EC 1.2.4.4 | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
reconstitution with lipoylated recombinant E2 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | - |
Rattus norvegicus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? | |
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] | phosphorylation inactivates EC 1.2.4.4 | Rattus norvegicus | ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0542 | - |
phosphate | 25°C, recombinant enzyme alone | Rattus norvegicus | |
0.475 | - |
phosphate | 25°C, reconstituted with lipoylated recombinant E2 | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | native PAGE | - |
8.5 |