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Literature summary for 2.7.11.4 extracted from

  • Shimomura, Y.; Nanaumi, N.; Suzuki, M.; Popov, K.M.; Harris, R.A.
    Purification and partial characterization of branched-chain alpha-ketoacid dehydrogenase kinase from rat liver and rat heart (1990), Arch. Biochem. Biophys., 283, 293-299.
    View publication on PubMed
Search for more literature for 2.7.11.4

Inhibitors

Inhibitors Comment Organism Structure
2-Chloroisohexanoate 50% inhibition at 0.014 mM, no inhibition with histone II-S as substrate Rattus norvegicus
Dichloroacetate 50% inhibition at 1.8 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Rattus norvegicus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Rattus norvegicus Sprague-Dawley phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
from purified branched-chain alpha-keto acid dehydrogenase complex Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0268
-
 liver enzyme Rattus norvegicus
0.0357
-
 heart enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
labile enzyme, best stored at -70°C in the presence of DTT Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone II-S
-
 Rattus norvegicus ?
-
 ?
ATP + histone II-S
-
 Rattus norvegicus Sprague-Dawley ?
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)]
-
 Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)]
-
 Rattus norvegicus Sprague-Dawley ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Rattus norvegicus Sprague-Dawley ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus