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Literature summary for 2.7.11.4 extracted from

  • Espinal, J.; Beggs, M.; Randle, P.J.
    Assay of branched-chain alpha-keto acid dehydrogenase kinase in mitochondrial extracts and purified branched-chain alpha-keto acid dehydrogenase complexes (1988), Methods Enzymol., 166, 166-175.
    View publication on PubMed
Search for more literature for 2.7.11.4

General Stability

General Stability Organism
precipitation of branched-chain oxo acid dehydrogenase enzyme complex at acid pH-values, especially below 6.5, results in specific loss of kinase activity Rattus norvegicus
precipitation of branched-chain oxo acid dehydrogenase enzyme complex at acid pH-values, especially below 6.5, results in specific loss of kinase activity Bos taurus
precipitation of branched-chain oxo acid dehydrogenase enzyme complex at acid pH-values, especially below 6.5, results in specific loss of kinase activity Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
2-(N-Morpholino)propane sulfonate buffer
-
 Bos taurus
2-(N-Morpholino)propane sulfonate buffer
-
 Oryctolagus cuniculus
2-(N-Morpholino)propane sulfonate buffer
-
 Rattus norvegicus
2-oxohexanedioate
-
 Bos taurus
2-oxohexanedioate
-
 Oryctolagus cuniculus
2-oxohexanedioate
-
 Rattus norvegicus
2-oxopentanoate
-
 Bos taurus
2-oxopentanoate
-
 Oryctolagus cuniculus
acetoacetyl-CoA
-
 Bos taurus
acetoacetyl-CoA
-
 Oryctolagus cuniculus
acetoacetyl-CoA
-
 Rattus norvegicus
ADP
-
 Bos taurus
ADP
-
 Oryctolagus cuniculus
ADP
-
 Rattus norvegicus
methylmalonyl-CoA
-
 Bos taurus
methylmalonyl-CoA
-
 Oryctolagus cuniculus
methylmalonyl-CoA
-
 Rattus norvegicus
n-Octanoate
-
 Bos taurus
n-Octanoate
-
 Oryctolagus cuniculus
n-Octanoate
-
 Rattus norvegicus
thiamine diphosphate
-
 Bos taurus
thiamine diphosphate
-
 Oryctolagus cuniculus
thiamine diphosphate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 ATP pH 7.5, 30°C Bos taurus
0.013
-
 ATP ATP in form of MgATP2- Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-
mitochondrion
-
 Bos taurus 5739
-
mitochondrion
-
 Oryctolagus cuniculus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement, actual substrate: MgATP2- Rattus norvegicus
Mg2+ requirement, actual substrate: MgATP2- Bos taurus
Mg2+ requirement, actual substrate: MgATP2- Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Rattus norvegicus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Bos taurus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] Oryctolagus cuniculus phosphorylation inactivates EC 1.2.4.4 ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from liver and heart, homogeneity Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
adipocyte
-
 Rattus norvegicus
-
heart
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
kidney
-
 Bos taurus
-
kidney
-
 Oryctolagus cuniculus
-
liver
-
 Rattus norvegicus
-
liver
-
 Bos taurus
-
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 various assay methods Rattus norvegicus
additional information
-
 various assay methods Bos taurus
additional information
-
 various assay methods Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)]
-
 Bos taurus ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] 2 Ser-residues in E1-alpha-subunit Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] 2 Ser-residues in E1-alpha-subunit Bos taurus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] 2 Ser-residues in E1-alpha-subunit Oryctolagus cuniculus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Rattus norvegicus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Bos taurus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?
ATP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphorylation inactivates EC 1.2.4.4 Oryctolagus cuniculus ADP + [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.27
-
 ADP pH 7.5, 30°C Bos taurus