Literature summary for 2.7.11.32 extracted from

Astley, H.; Parsley, K.; Aubry, S.; Chastain, C.; Burnell, J.; Webb, M.; Hibberd, J.
The pyruvate, orthophosphate dikinase regulatory proteins of Arabidopsis are both bifunctional and interact with the catalytic and nucleotide-binding domains of pyruvate, orthophosphate dikinase (2011), Plant J., 68, 1070-1080.

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Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged protein expressed in Escherichia coli BL21(DE3)pLysS	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	many chimeric proteins (regulatory protein 1/regulatory protein 2) are tested for kinase activity and interaction with the substrate protein	Arabidopsis thaliana
additional information	many chimeric proteins (regulatory protein 1/regulatory protein 2) are tested for kinase activity and interaction with the substrate protein, 21 site-directed mutant proteins (conserved amino acid residues) are tested for kinase activity and/or interaction with the substrate protein	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-
cytoplasm	-	Arabidopsis thaliana	5737	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-
Arabidopsis thaliana	O23404	cf. EC 2.7.9.1	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography (Ni2+)	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate, phosphate dikinase + ADP	active	Arabidopsis thaliana	[pyruvate, phosphate dikinase] phosphate + AMP	inactive	?

Synonyms

Synonyms	Comment	Organism
PPDK regulatory protein 1	-	Arabidopsis thaliana
PPDK regulatory protein 2	-	Arabidopsis thaliana
pyruvate, orthophosphate dikinase regulatory protein 1	bifunctional	Arabidopsis thaliana
pyruvate, orthophosphate dikinase regulatory protein 2	bifunctional	Arabidopsis thaliana

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Release 2023.1 (January 2023)