General Stability | Organism |
---|---|
highly labile enzyme | Rattus norvegicus |
protease inhibitors stabilizes enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | - |
Rattus norvegicus | |
Inhibitor W-7 | specific Ca2+/calmodulin-dependent kinase inhibitor | Rattus norvegicus | |
Trifluperazine | specific Ca2+/calmodulin-dependent kinase inhibitor | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00085 | - |
hydroxymethylglutaryl-CoA reductase | pH 6.5, 30°C, low-MW kinase | Rattus norvegicus | |
0.0029 | - |
histone H1 | pH 6.5, 30°C, low-MW kinase | Rattus norvegicus | |
0.028 | - |
ATP | pH 6.5, 30°C, low-MW kinase | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | 125000 | low-MW kinase, gel filtration | Rattus norvegicus |
560000 | 600000 | high-MW kinase, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] | Rattus norvegicus | inactivates EC 1.1.1.34 by phosphorylation | ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley | - |
Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Comment) | Organism |
---|---|
low-MW kinase | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.292 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | relative kinase activity for low-MW kinase 8%, high MW-kinase 48% | Rattus norvegicus | ADP + casein phosphate | - |
? | |
ATP + casein | relative kinase activity for low-MW kinase 8%, high MW-kinase 48% | Rattus norvegicus Sprague-Dawley | ADP + casein phosphate | - |
? | |
ATP + dephospho-alpha,beta-tubulin | relative kinase activity high MW-kinase 15% | Rattus norvegicus | ADP + [alpha,beta-tubulin] phosphate | - |
? | |
ATP + glycogen synthase | relative kinase activity for low-MW kinase 7%, high MW-kinase 87% | Rattus norvegicus | ADP + [glycogen synthase] phosphate | - |
? | |
ATP + heavy meromyosin | relative kinase activity for low-MW kinase 2%, high MW-kinase 100% | Rattus norvegicus | ADP + [heavy meromyosin] phosphate | - |
? | |
ATP + histone H1 | - |
Rattus norvegicus | ADP + phosphohistone H1 | - |
? | |
ATP + histone H1 (IIIS) | histones are better substrates for high-MW kinase than hydroxymethylglutaryl-CoA reductase, relative kinase activity for low-MW kinase 275%, high MW-kinase 103% | Rattus norvegicus | ADP + [histone H1 (IIIS)] phosphate | - |
? | |
ATP + histone II-S | relative kinase activity for low-MW kinase 38%, high MW-kinase 159% | Rattus norvegicus | ADP + [histone II-S] phosphate | - |
? | |
ATP + histone VIIIS | relative kinase activity for low-MW kinase 65%, high MW-kinase 141% | Rattus norvegicus | ADP + [histone VIIIS] phosphate | - |
? | |
ATP + MAP-2 | relative kinase activity for low-MW kinase 14%, high MW-kinase 566% | Rattus norvegicus | ADP + MAP-2 phosphate | - |
? | |
ATP + myelin basic protein | moderate substrate for low-MW kinase, better than hydroxymethylglutaryl-CoA reductase for high-MW kinase, relative kinase activity for low-MW kinase 36%, high MW-kinase 238% | Rattus norvegicus | ADP + [myelin basic protein] phosphate | - |
? | |
ATP + myosin mixed light chains | relative kinase activity for low-MW kinase 4%, high MW-kinase 27% | Rattus norvegicus | ADP + [myosin mixed light chains] phosphate | - |
? | |
ATP + phosphorylase B | relative kinase activity high MW-kinase 12% | Rattus norvegicus | ADP + [phosphorylase B] phosphate | - |
? | |
ATP + phosvitin | relative kinase activity for low-MW kinase 2%, high MW-kinase 2% | Rattus norvegicus | ADP + phosvitin phosphate | - |
? | |
ATP + phosvitin | relative kinase activity for low-MW kinase 2%, high MW-kinase 2% | Rattus norvegicus Sprague-Dawley | ADP + phosvitin phosphate | - |
? | |
ATP + protamine | relative kinase activity for low-MW kinase 24%, high MW-kinase 38% | Rattus norvegicus | ADP + protamine phosphate | - |
? | |
ATP + protamine | relative kinase activity for low-MW kinase 24%, high MW-kinase 38% | Rattus norvegicus Sprague-Dawley | ADP + protamine phosphate | - |
? | |
ATP + synapsin 1 | as good substrate as hydroxymethylglutaryl-CoA reductase, relative kinase activity for low-MW kinase 151%, high MW-kinase 103% | Rattus norvegicus | ADP + [synapsin 1] phosphate | - |
? | |
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] | - |
Rattus norvegicus | ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate | - |
? | |
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] | inactivates EC 1.1.1.34 by phosphorylation | Rattus norvegicus | ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate | - |
? | |
additional information | autophosphorylation in absence of substrate | Rattus norvegicus | ? | - |
? | |
additional information | incorporates 0.5 mol phosphate/mol MW 53000 enzyme substrate fragment, 2 mol phosphate/mol native enzyme substrate | Rattus norvegicus | ? | - |
? | |
additional information | autophosphorylation in absence of substrate | Rattus norvegicus Sprague-Dawley | ? | - |
? | |
additional information | incorporates 0.5 mol phosphate/mol MW 53000 enzyme substrate fragment, 2 mol phosphate/mol native enzyme substrate | Rattus norvegicus Sprague-Dawley | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
low-MW kinase | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | about half-maximal activity at pH 5.5 and 7.0, low-MW kinase | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | requirement, Ca2+/calmodulin dependent kinase, no phosphorylation of substrate observed in absence | Rattus norvegicus |