Literature summary for 2.7.11.30 extracted from

Harrison, C.A.; Gray, P.C.; Koerber, S.C.; Fischer, W.; Vale, W.
Identification of a functional binding site for activin on the type I receptor ALK4 (2003), J. Biol. Chem., 278, 21129-21135.

Search for more literature for 2.7.11.30

Activating Compound

Activating Compound	Comment	Organism	Structure
actividin A	required for activity in a complex formed with ALK4 and ActRIIB or ActRII, identification of the functional binding site	Homo sapiens
actividin B	required for activity in a complex formed with ALK4 and ActRIIB or ActRII, identification of the functional binding site	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
surface expression of wild-type and mutant ALK4 type I receptors in HEK293T cells, expression of wild-type and mutant ALK4 type I receptors in mink lung epithelial cells, Mv1Lu cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A78G	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
D89A	site-directed mutagenesis, the mutation does not affect binding of actividin	Homo sapiens
E74A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
E88A	site-directed mutagenesis, the mutation does not affect binding of actividin	Homo sapiens
F82A	site-directed mutagenesis, the mutation slightly affects binding of actividin	Homo sapiens
G79A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
I70A	site-directed mutagenesis, the mutation of a residue from the ALK4 extracellular domain affects the binding of activin and the substantial effects of the dominant negative truncated ALK4 mutant	Homo sapiens
K72A	site-directed mutagenesis, the mutation does not affect binding of actividin	Homo sapiens
K80A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
L40A	site-directed mutagenesis, the mutation of a residue from the ALK4 extracellular domain affects the binding of activin and the substantial effects of the dominant negative truncated ALK4 mutant	Homo sapiens
L75A	site-directed mutagenesis, the mutation of a residue from the ALK4 extracellular domain affects the binding of activin and the substantial effects of the dominant negative truncated ALK4 mutant	Homo sapiens
L85A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
L90A	site-directed mutagenesis, the mutation does not affect binding of actividin	Homo sapiens
M53A	site-directed mutagenesis, the mutation slightly affects binding of actividin	Homo sapiens
additional information	construction of a truncated dominant negative ALK4 mutant	Homo sapiens
P71A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
P77A	site-directed mutagenesis, the mutation of a residue from the ALK4 extracellular domain affects the binding of activin and the substantial effects of the dominant negative truncated ALK4 mutant	Homo sapiens
P81A	site-directed mutagenesis, the mutation does not affect binding of actividin	Homo sapiens
R91A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
S38A	site-directed mutagenesis, the mutation slightly affects binding of actividin	Homo sapiens
S55A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
S86A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
S87A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
T93A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
V73A	site-directed mutagenesis, the mutation of a residue from the ALK4 extracellular domain affects the binding of activin and the substantial effects of the dominant negative truncated ALK4 mutant	Homo sapiens
V76A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens
Y83A	site-directed mutagenesis, the mutation affects binding of actividin	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	transmembrane protein	Homo sapiens	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	ALK4 forms a complex with type II serine/threonine transmembrane receptor ActRIIB or ActRII and activin for initiation of signaling, ALK3 forms a complex with bone morphogenetic protein-2	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	ALK4 is a type I transmembrane serine/threonine kinase receptor	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
bone	ALK3	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	ALK4 forms a complex with type II serine/threonine transmembrane receptor ActRIIB or ActRII and activin for initiation of signaling, ALK3 forms a complex with bone morphogenetic protein-2	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	ALK3 crystal structure model analysis	Homo sapiens

Synonyms

Synonyms	Comment	Organism
activin-like kinase receptor 4	-	Homo sapiens
ALK4	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens