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Literature summary for 2.7.11.26 extracted from

  • Tugaeva, K.V.; Tsvetkov, P.O.; Sluchanko, N.N.
    Bacterial co-expression of human Tau protein with protein kinase A and 14-3-3 for studies of 14-3-3/phospho-Tau interaction (2017), PLoS ONE, 12, e0178933 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.26

Cloned(Commentary)

Cloned (Comment) Organism
the enzyme and human fetal tau-protein are coexpressed in Escherichia coli BL21(DE3) cells Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 4 mM used in assay conditions Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + HSPB6 phosphorylation at Ser16 Mus musculus ADP + phosphorylated HSPB6
-
 ?
ATP + tau-protein good substrate for protein kinase A which phosphorylates several key residues (Ser156, Ser235, Ser267, Ser320, Ser327) of tau-protein and induces its tight interaction with 14-3-3 proteins Mus musculus ADP + O-phospho-tau-protein
-
 ?
additional information often protein kinase A works in pair with 14-3-3 since it phosphorylates target proteins at (R/K)RX(S/T) sequences (where X is any amino acid) Mus musculus ?
-
-

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Mus musculus
? x * 44900, calculated from amino acid sequence Mus musculus

Synonyms

Synonyms Comment Organism
PKA
-
 Mus musculus
protein kinase A
-
 Mus musculus