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Literature summary for 2.7.11.26 extracted from
- A noncatalytic domain of glycogen synthase kinase-3 (GSK-3) is essential for activity (2010), J. Biol. Chem., 285, 7957-7963.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient transfection of GST-tagged enzyme into HEK-293 cells coexpression with recombinant tau, coexpression of wild-type GSK-3alpha and wild-type GSK-3beta enhances Tau phosphorylation at Ser396 and Ser404 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K148R | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
| K85R | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
| additional information | deletion of the C-terminus to result in mutants alphaDELTACT1-4, with remaining 448, 441, 430, and 416 residues of 483, respectively, leads to loss of nearly all activity of the GSK-3 isoform and abolishes suppression of Wnt signaling | Homo sapiens |
| additional information | deletion of the C-terminus to result in mutants betaDELTACT1-4, with remaining 385, 378, 367, and 344 residues of 420, respectively, leads to loss of nearly all activity of the GSK-3 isoform and abolishes suppression of Wnt signaling | Homo sapiens |
| P379A/P380A | site-directed mutagenesis, the mutant shows rediuced activity compared to the wild-type | Homo sapiens |
| P442A/P443A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
| R159A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
| R96A | site-directed mutagenesis, the mutant activity is similar to the wild-type | Homo sapiens |
| S9A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type | Homo sapiens |
| T390A | site-directed mutagenesis, the mutant activity is similar to the wild-type | Homo sapiens |
| Y216F | site-directed mutagenesis, the mutant activity is similar to the wild-type | Homo sapiens |
| Y279F | site-directed mutagenesis, the mutant activity is similar to the wild-type | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [tau-protein] | Homo sapiens | - |
ADP + O-phospho-[tau-protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P49840 | isozyme alpha | - |
| Homo sapiens | P49841 | isozyme beta | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from HEK-293 cells by glutathione affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [tau-protein] | - |
Homo sapiens | ADP + O-phospho-[tau-protein] | - |
? | |
| additional information | GSK-3alpha-specific requirement for priming of tau protein | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogen synthase kinase-3 | - |
Homo sapiens |
| GSK-3 | - |
Homo sapiens |
| GSK-3alpha | - |
Homo sapiens |
| GSK-3beta | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | deletion of the C-terminus alphaDELTACT-4 leads to loss of nearly all activity of the GSK-3 isoform and abolishes suppression of Wnt signaling, GSK-3 C-terminal deletions mutants fail to interact with axin GID | Homo sapiens |
| malfunction | deletion of the C-terminus betaDELTACT-4 leads to loss of nearly all activity of the GSK-3 isoform and abolishes suppression of Wnt signaling, GSK-3 C-terminal deletions mutants fail to interact with axin GID | Homo sapiens |
| additional information | amino acids 345367 of GSK-3beta are essential for enzyme activity with tau protein, structure-function analysis of GSK-3alpha and GSK-3beta in mammalian cells | Homo sapiens |
| additional information | amino acids 417-430 of GSK-3alpha are essential for enzyme activity with tau protein, structure-function analysis of GSK-3alpha and GSK-3beta in mammalian cells | Homo sapiens |
| physiological function | glycogen synthase kinase-3 (GSK-3) isoforms, GSK-3alpha and GSK-3beta, are serine/threonine kinases involved in numerous cellular processes and diverse diseases, including Alzheimer disease, cancer, and diabetes. GSK-3 isoforms function redundantly in some settings, while, in others, they exhibit distinct activities. In the canonical Wnt signaling pathway, GSK-3 phosphorylation of beta-catenin is regulated via interactions with the scaffold protein axin | Homo sapiens |
| physiological function | in the canonical Wnt signaling pathway, GSK-3 phosphorylation of beta-catenin is regulated via interactions with the scaffold protein axin. Glycogen synthase kinase-3 (GSK-3) isoforms, GSK-3alpha and GSK-3beta, are serine/threonine kinases involved in numerous cellular processes and diverse diseases, including Alzheimer disease, cancer, and diabetes. GSK-3 isoforms function redundantly in some settings, while, in others, they exhibit distinct activities. In the canonical Wnt signaling pathway, GSK-3 phosphorylation of beta-catenin is regulated via interactions with the scaffold protein axin | Homo sapiens |
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Release 2023.1 (January 2023)
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