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Literature summary for 2.7.11.26 extracted from

  • Sato, S.; Cerny, R.L.; Buescher, J.L.; Ikezu, T.
    Tau-tubulin kinase 1 (TTBK1), a neuron-specific tau kinase candidate, is involved in tau phosphorylation and aggregation (2006), J. Neurochem., 98, 1573-1584.
    View publication on PubMed

Application

Application Comment Organism
medicine TTBK1 is located on human chromosome 6p21.1. It is a neuron-specific dual kinase involved in tau phosphorylation at Alzheimer’s disease-related sites and is also associated with tau aggregation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
HA- and myc-tagged full-length TTBK1 expressed in COS-7 cells, HA-TTBK1 expressed in HEK293 cells and in mock-transfected cells, recombinant full length TTBK1 expressed in Escherichia coli or baculovirus-infected Sf-9 insect cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Mus musculus 5737
-
additional information perinuclear region Mus musculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens
Mn2+
-
Homo sapiens
additional information not activated by K+, Ca2+ or Zn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
absence of a 230-kDa protein in the mouse cortex, suggests a very small quantity of full-length TTBK1 Mus musculus
100000
-
immunoprecipitation, lysosomal degradation product of full-length TTBK1 Mus musculus
100000
-
immunoprecipitation, lysosomal degradation product of full-length TTBK1 Homo sapiens
142700
-
predicted Homo sapiens
230000
-
immunoprecipitation, full-length TTBK1 migrates to 230 kDa because of the negative charge of the polyglutamate sequence and the protein modification Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
adult C57/BL6 mice
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin and by gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain adult brain cortex and fetal brain tissue Homo sapiens
-
brain cortical layers Mus musculus
-
cerebellum
-
Homo sapiens
-
cerebellum granular layer Mus musculus
-
hippocampus CA1 layer Mus musculus
-
additional information perinuclear and cytoplasmic regions of the large cortical pyramidal cells in the temporal cortex Mus musculus
-
neuron
-
Homo sapiens
-
neuron CA1 pyramidal neurons and cerebellum granular neurons Mus musculus
-
spinal cord weak expression Homo sapiens
-
testis weak expression Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.26
-
in the presence of Mg2+ Homo sapiens
0.57
-
in the presence of Mn2+ Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protein tau direct tau phosphorylation by TTBK1 at Ser198, Ser199, Ser202 and Ser422 Homo sapiens ADP + protein tau phosphate
-
?

Synonyms

Synonyms Comment Organism
Tau-tubulin kinase 1
-
Mus musculus
Tau-tubulin kinase 1
-
Homo sapiens
TTBK1
-
Mus musculus
TTBK1
-
Homo sapiens