Application | Comment | Organism |
---|---|---|
medicine | TTBK1 is located on human chromosome 6p21.1. It is a neuron-specific dual kinase involved in tau phosphorylation at Alzheimers disease-related sites and is also associated with tau aggregation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
HA- and myc-tagged full-length TTBK1 expressed in COS-7 cells, HA-TTBK1 expressed in HEK293 cells and in mock-transfected cells, recombinant full length TTBK1 expressed in Escherichia coli or baculovirus-infected Sf-9 insect cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Mus musculus | 5737 | - |
additional information | perinuclear region | Mus musculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens | |
additional information | not activated by K+, Ca2+ or Zn2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
absence of a 230-kDa protein in the mouse cortex, suggests a very small quantity of full-length TTBK1 | Mus musculus |
100000 | - |
immunoprecipitation, lysosomal degradation product of full-length TTBK1 | Mus musculus |
100000 | - |
immunoprecipitation, lysosomal degradation product of full-length TTBK1 | Homo sapiens |
142700 | - |
predicted | Homo sapiens |
230000 | - |
immunoprecipitation, full-length TTBK1 migrates to 230 kDa because of the negative charge of the polyglutamate sequence and the protein modification | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
adult C57/BL6 mice | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin and by gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | adult brain cortex and fetal brain tissue | Homo sapiens | - |
brain | cortical layers | Mus musculus | - |
cerebellum | - |
Homo sapiens | - |
cerebellum | granular layer | Mus musculus | - |
hippocampus | CA1 layer | Mus musculus | - |
additional information | perinuclear and cytoplasmic regions of the large cortical pyramidal cells in the temporal cortex | Mus musculus | - |
neuron | - |
Homo sapiens | - |
neuron | CA1 pyramidal neurons and cerebellum granular neurons | Mus musculus | - |
spinal cord | weak expression | Homo sapiens | - |
testis | weak expression | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.26 | - |
in the presence of Mg2+ | Homo sapiens |
0.57 | - |
in the presence of Mn2+ | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein tau | direct tau phosphorylation by TTBK1 at Ser198, Ser199, Ser202 and Ser422 | Homo sapiens | ADP + protein tau phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tau-tubulin kinase 1 | - |
Mus musculus |
Tau-tubulin kinase 1 | - |
Homo sapiens |
TTBK1 | - |
Mus musculus |
TTBK1 | - |
Homo sapiens |