Protein Variants | Comment | Organism |
---|---|---|
additional information | identification of a Raf11 knockout mutant, raf11 (SALK_047070), the T-DNA is located in the ninth exon of the Raf11 gene, and Raf11 expression is abolished completely. Contruction of a Raf10 and Raf11 double knockout mutant line, ais143 raf11, by crossing ais143 with raf11, phenotypes, overview | Arabidopsis thaliana |
additional information | isolation of the abscisic acid-insensitive mutant, ais143, by activation tagging screen. The T-DNA is located in one of the exons (8/13) of the coding region of putative mitogen-activated protein (MAP) kinase kinase kinase (MAP3K) gene, Raf10, thereby abolishing its expression in the mutant. ais143 exhibits reduced seed dormancy as well as reduced abscisic acid sensitivity. The phenotypes are complemented by the wild-type Raf10 gene, and the overexpression of Raf10 results in delayed seed germination and enhanced the abscisic acid sensitivity | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BAY 43-9006 | - |
Arabidopsis thaliana | |
additional information | no inhibition of Raf10 by the MAP2K inhibitor U0126; no inhibition of Raf11 by the MAP2K inhibitor U0126 | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | F4HVH9 | - |
- |
Arabidopsis thaliana | F4K645 | - |
- |
Arabidopsis thaliana Col-0 | F4HVH9 | - |
- |
Arabidopsis thaliana Col-0 | F4K645 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Arabidopsis thaliana | - |
flower | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
seedling | Raf11 promoter activity is observed in embryos and most of the seedling tissues, except emerging leaves and root tips, Raf11 expression patterns, overview | Arabidopsis thaliana | - |
silique | abscission zones | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Raf10 has autophosphorylation activity | Arabidopsis thaliana | ? | - |
? | |
additional information | Raf11 has autophosphorylation activity | Arabidopsis thaliana | ? | - |
? | |
additional information | Raf11 has autophosphorylation activity | Arabidopsis thaliana Col-0 | ? | - |
? | |
additional information | Raf10 has autophosphorylation activity | Arabidopsis thaliana Col-0 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAP kinase kinase kinase | - |
Arabidopsis thaliana |
MAP3K | - |
Arabidopsis thaliana |
Raf10 | - |
Arabidopsis thaliana |
Raf11 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | Raf10 expression in seedlings is induced by ABA, salt, mannitol and cold treatment | up |
Arabidopsis thaliana | Raf11 expression in seedlings is induced by ABA, salt, mannitol and cold treatment | up |
General Information | Comment | Organism |
---|---|---|
malfunction | the abscisic acid-insensitive mutant, ais143, located in the coding region of MAP3K gene Raf10, abolishes RAF10 expression in the mutant. ais143 exhibits reduced seed dormancy as well as reduced abscisic acid sensitivity. The phenotypes are complemented by the wild-type Raf10 gene, and the overexpression of Raf10 results in delayed seed germination and enhanced the abscisic acid sensitivity. Raf11 knockout and overexpression lines show that their phenotypes are similar to those of Raf10 knockout and overexpression lines. An ais143 raf11 double mutant exhibits stronger phenotypes than single mutants, indicating the functional redundancy between Raf10 and Raf11. Expression of many abscisic acid-associated genes, including the key regulatory genes ABI3 and ABI5, is altered in the Raf10 and Raf11 overexpressing lines. Reduced seed dormancy of ais143 and raf11, overview. Both germination and cotyledon greening of ais143 and ais143 raf11 plants are less sensitive to high salt compared to wild-type | Arabidopsis thaliana |
physiological function | mitogen-activated protein (MAP) kinase cascades are universal signaling modules in eukaryotes, which relay external or internal upstream signals to downstream targets. Arabidopsis thaliana putative MAP kinase kinase kinases Raf10 and Raf11 are positive regulators of seed dormancy and abscisic acid response | Arabidopsis thaliana |
physiological function | mitogen-activated protein (MAP) kinase cascades are universal signaling modules in eukaryotes, which relay external or internal upstream signals to downstream targets. Arabidopsis thaliana putative MAP kinase kinase kinases Raf10 and Raf11 are positive regulators of seed dormancy. Raf11 plays a redundant role in abscisic acid response | Arabidopsis thaliana |