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Literature summary for 2.7.11.25 extracted from

  • Lafleur, E.; Kapfer, C.; Joly, V.; Liu, Y.; Tebbji, F.; Daigle, C.; Gray-Mitsumune, M.; Cappadocia, M.; Nantel, A.; Matton, D.P.
    The FRK1 mitogen-activated protein kinase kinase kinase (MAPKKK) from Solanum chacoense is involved in embryo sac and pollen development (2015), J. Exp. Bot., 66, 1833-1843.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ScFRK1, DNA and amino acid sequence determination and analysis, phylogenetic analysis. for sense and antisense constructs, the GFP-tagged ScFRK1 cDNA is inserted in a modified pBin19 transformation vector with a Cauliflower mosaic virus (CaMV) 35S double enhancer promoter. Sense and antisense constructs are individually transformed in Agrobacterium tumefaciens LBA4404 by electroporation. Solanum chacoense plants are transformed by the leaf disc method. The ScFRK1-GFP fusion protein is restricted to the nuclei and co-localized with the 4',6-diamidino-2-phenylindole (DAPI) signal. Tissue expression patterns, overview Solanum chacoense

Protein Variants

Protein Variants Comment Organism
additional information construction of the ScFRK1DELTA1 or ScFRK1DELTA1DELTA2 mutants that are localized in the cytoplasm in contrast to the wild-type. Mutant phenotypes, overview Solanum chacoense

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information analysis of cellular localization of the ScFRK1 kinase, overview Solanum chacoense
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nucleus
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Solanum chacoense 5634
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7000
-
-
Solanum chacoense

Organism

Organism UniProt Comment Textmining
Solanum chacoense T2B355
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Source Tissue

Source Tissue Comment Organism Textmining
anther
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Solanum chacoense
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embryo
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Solanum chacoense
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flower bud
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Solanum chacoense
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gametophyte female Solanum chacoense
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leaf
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Solanum chacoense
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megaspore mother cell Solanum chacoense
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additional information enzyme FRK1 shows a highly specific expression profile following fertilization. The ScFRK1 gene is expressed early on during female gametophyte development in the integument and megaspore mother cell and, later, in the synergid and egg cells of the embryo sac. Strong ScFRK1 mRNA accumulation is only observed in the ovary and, to a lesser extent, in the style. ScFRK1 is expressed in both the sporophyte and the gametophyte. No ScFRK1 mRNA signal is detected in mature pollen, but a strong signal is observed in crosssections of flower buds in the anthers. Expression patterns, overview Solanum chacoense
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plant embryo
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Solanum chacoense
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plant ovule
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Solanum chacoense
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pollen mother cell
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Solanum chacoense
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style
-
Solanum chacoense
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synergid cell
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Solanum chacoense
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Subunits

Subunits Comment Organism
? x * 7000, about, sequence calculation Solanum chacoense
More a short C-terminal region of 42 amino acids (position 282--323) follows kinase subdomain XI, presence of a cluster of two short basic amino acid sequences predicted to form a bipartite nucleoplasmin-type nuclear localization sequence (NLS) Solanum chacoense

Synonyms

Synonyms Comment Organism
fertilization-related kinase 1
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Solanum chacoense
Frk1
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Solanum chacoense
MAPKKK
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Solanum chacoense
mitogen-activated protein kinase kinase kinase
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Solanum chacoense
ScFRK1
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Solanum chacoense

General Information

General Information Comment Organism
evolution enzyme FRK1 belongs to a small group of pMEKKs that do not possess an extended N- or C-terminal regulatory domain Solanum chacoense
malfunction transgenic plants with lower or barely detectable levels of ScFRK1 mRNAs show production of small fruits with severely reduced seed set, resulting from a concomitant decline in the number of normal embryo sacs produced. Megagametogenesis and microgametogenesis are affected, as megaspores do not progress beyond the functional megaspore (FG1) stage and the microspore collapses around the first pollen mitosis. As for other mutants that affect embryo sac development, pollen tube guidance is severely affected in the ScFRK1 transgenic lines. Gametophyte to sporophyte communication is also affected, as observed from a marked change in the transcriptomic profiles of the sporophytic tissues of the ovule Solanum chacoense
physiological function the FRK1 mitogen-activated protein kinase kinase kinase (MAPKKK) from Solanum chacoense is involved in embryo sac and pollen development, the enzyme is involved in a signalling cascade that regulates both male and female gamete development Solanum chacoense