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Literature summary for 2.7.11.25 extracted from
- Identification and functional analysis of mitogen-activated protein kinase kinase kinase (MAPKKK) genes in canola (Brassica napus L.) (2014), J. Exp. Bot., 65, 2171-2188.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| screening for MAPKKK genes and cloning of 28 genes, DNA and amino acid sequence determination and analysis, phylogenetic analysis, clonig of 28 genes comprising four MAPKKKs, seven ZIKs, and 17 Raf genes, overview | Brassica napus |
| screening for MAPKKK genes and cloning of 28 genes, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time reverse transcription-PCR expression analysis, transient recombinant expression of gene BnaMAPKKK18.1 as YFP- or GFP-tagged protein in Nicotiana benthamiana leaves via transfection method using Agrobacterium tumefaciens strain GV3101 | Brassica napus |
| screening for MAPKKK genes and cloning of 28 genes, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time reverse transcription-PCR expression analysis, transient recombinant expression of gene BnaMAPKKK19.1 as YFP- or GFPtagged protein in Nicotiana benthamiana leaves via transfection method using Agrobacterium tumefaciens strain GV3101 | Brassica napus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K32G | site-directed mutagenesis | Brassica napus |
| K37G | site-directed mutagenesis | Brassica napus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Brassica napus | 9507 | - |
| cytoplasm | - |
Brassica napus | 5737 | - |
| additional information | subcellular distribution of MAPKKK proteins, overview | Brassica napus | - |
- |
| nucleus | - |
Brassica napus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Brassica napus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | Brassica napus | - |
ADP + a phosphoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | - |
double haploid DH12075, diverse MAPKKK genes | - |
| Brassica napus | V9LY75 | gene MAPKKK19.1; double haploid DH12075 | - |
| Brassica napus | V9LYK6 | gene MAPKKK18.1; double haploid DH12075 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Brassica napus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | - |
Brassica napus | ADP + a phosphoprotein | - |
? | |
| additional information | identification and validation of BnaMAPKKK and BnaMKK interactions. BnaMKK1 and BnaMKK4 do not interact with any of the 28 BnaMAPKKK proteins assayed. Six BnaMAPKKKs, BnaMAPKKK17, -18, and -20, BnaZIK3 and -4, and BnaRaf35 interact with BnaMKK3. Six BnaMAPKKKs, BnaMAPKKK17, -19, -20, BnaZIK2, -9, and BnaRaf28, interact with BnaMKK9. Three BnaMAPKKKs, BnaZIK2, -9, and BnaRaf28, interact with BnaMKK5, and five BnaMAPKKKs, BnaMAPKKK17, -19, -20, BnaZIK2, and BnaRaf28 interact with BnaMKK8. As regards BnaMKK2 and -6, only BnaRaf28 interact with both of them | Brassica napus | ? | - |
? | |
| additional information | identification and validation of BnaMAPKKK and BnaMKK interactions. No interactions with BnaMKK1, BnaMKK2, BnaMKK4, BnaMKK5, BnaMKK6, BnaMKK8, and BnaMKK9. BnaMAPKKK18 interacts with MKK3 | Brassica napus | ? | - |
? | |
| additional information | identification and validation of BnaMAPKKK and BnaMKK interactions. No interactions with BnaMKK1-BnaMKK6. BnaMAPKKK19 interacts with BnaMKK8 and with BnaMKK9 wild-type and mutants S193D/S199E and K74R. BnaMKK9S193D/S199E is constitutively active while the K74R mutant is constitutively inactive | Brassica napus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BnaM3K18 | - |
Brassica napus |
| BnaM3K19 | - |
Brassica napus |
| BnaMAPKKK | - |
Brassica napus |
| BnaMAPKKK18.1 | - |
Brassica napus |
| BnaMAPKKK19.1 | - |
Brassica napus |
| MAPKKK | - |
Brassica napus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Brassica napus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Brassica napus | diverse MAPKKK enzymes, equence calculations, overview | 9.42 | 4.74 |
| Brassica napus | sequence calculation | - |
5.02 |
| Brassica napus | sequence calculation | - |
5.03 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Brassica napus | 1-aminocyclopropane-1-carboxylic acid decreases the expression of BnaMAPKKK18 | down |
| Brassica napus | jasmonic acid downregulates BnaZIK3, BnaZIK4, BnaRaf36, and BnaRaf39. 1-Aminocyclopropane-1-carboxylic acid represses BnaMAPKKK18, BnaZIK3, and BnaRaf36. Salicylic acid represses BnaZIK2, BnaRaf34, and BnaRaf36. Methyl viologen treatment downregulates the expression of BnaMAPKKK17, BnaRaf34, and BnaRaf36, while abscisic acid represses BnaZIK3 expression but exerts no effect on the transcription of the other genes. Salinity downregulates BnaMAPKKK20, BnaZIK3, BnaZIK8, and BnaRaf17, as well as BnaRaf29 and BnaRaf34. Expression of BnaZIK2, -3, -4, and BanRaf17 is decreased by heat treatment. Moderate cold stress at 4°C decreased the transcript abundance of BnaMAPKKK17, ZIK2, -3, -4, and BanRaf39. Challenge by the fungal pathogen Sclerotinia sclerotiorum represses the expression of BnaRaf28, -29, -30, and -36 | down |
| Brassica napus | challenge by the fungal pathogen Sclerotinia sclerotiorum and heat stress at 37°C induces the expression of BnaMAPKKK19 | up |
| Brassica napus | jasmonic acid induces BnaRaf30. 1-Aminocyclopropane-1-carboxylic acid induces BnaZIK8 and BnaRaf29. Salicylic acid significantly induces BnaMAPKKK18 and BnaRaf28. Salinity induces BnaMAPKKK20 and BnaRaf36. Heat treatment at 37°C induces BnaMAPKKK18, -19, and BnaRaf35 Moderate cold stress at 4°C increases the transcript abundance of BnaMAPKKK20 and BnaRaf29. Challenge by the fungal pathogen Sclerotinia sclerotiorum significantly induces BnaMAPKKK19, ZIK4, Raf34, -35, and -39. Oxalic acid slightly induces BnaMAPKKK20 | up |
| Brassica napus | salicylic acid and heat stress at 37°C induces the expression of BnaMAPKKK18 | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | gene BnaMAPKKK18 belongs to the MEKK subfamily of the MAPKKK family, phylogenetic and domain analysis of MAPKKK genes in Brassica napus and compaarison to MAPKKK enzymes from Arabidopsis thaliana and Oryza sativa, overview | Brassica napus |
| evolution | gene BnaMAPKKK19 belongs to the MEKK subfamily of the MAPKKK family, phylogenetic and domain analysis of MAPKKK genes in Brassica napus and compaarison to MAPKKK enzymes from Arabidopsis thaliana and Oryza sativa, overview | Brassica napus |
| evolution | subfamilyiesof the MAPKKK family, phylogenetic and domain analysis of MAPKKK genes in Brassica napus and compaarison to MAPKKK enzymes from Arabidopsis thaliana and Oryza sativa, overview | Brassica napus |
| metabolism | mitogen-activated protein kinase (MAPK) signalling cascades, consisting of three types of reversibly phosphorylated kinases (MAPKKK, MAPKK, and MAPK), are involved in important processes including plant immunity and hormone responses. The MAPKKKs comprise the largest family in the MAPK cascades | Brassica napus |
| physiological function | BnaMAPKKK plays a role in the cross-talk of multiple stresses, including both abiotic and biotic stresses, overview. BnaMAPKKK18 elicits hypersensitive response (HR)-like cell death when transiently expressed in Nicotiana benthamiana leaves | Brassica napus |
| physiological function | BnaMAPKKK plays a role in the cross-talk of multiple stresses, including both abiotic and biotic stresses, overview. BnaMAPKKK19 elicits hypersensitive response (HR)-like cell death when transiently expressed in Nicotiana benthamiana leaves. Gene BnaMAPKKK19 probably mediates cell death through MAPK kinase BnaMKK9. | Brassica napus |
| physiological function | MAPKKK functions in biotic and abiotic stress responses in Brassica napus, overview | Brassica napus |
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