Literature summary for 2.7.11.25 extracted from

Miao, Y.; Laun, T.M.; Smykowski, A.; Zentgraf, U.
Arabidopsis MEKK1 can take a short cut: it can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter (2007), Plant Mol. Biol., 65, 63-76.

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Application

Application	Comment	Organism
additional information	MEKK1 has a dual function, it can bind to the promoter of WRKY53 and it can interact with the WRKY53 on the protein level and can phosphorylate WRKY53 in vitro thereby increasing its DNA-binding activity. MEKK1 may be able to take a very direct short cut in MAPK signalling by directly phosphorylating a transcription factor	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
full-length cDNA inserted into pGBKT7 vector to generate a MEKK1-GBD fusion construct, introduced into yeast strain Y187 containing the LacZ gene under the control of the GAL1 promoter, full-length MEKK1 fragments subcloned into the pQE30 expression vector to create pQE-MEKK1 encoding the 6 x His-tagged protein and expressed in Escherichia coli, full-length MEKK1 cDNA fragment with a HA-tag cloned into the pGFPc155c vector encoding the C-terminal part of the GFP protein and transformed to Agrobacterium tumefaciens strain GV3101/pMP90 and injected to Arabidopsis leaves	Arabidopsis thaliana

Cloned (Comment)

Organism

full-length cDNA inserted into pGBKT7 vector to generate a MEKK1-GBD fusion construct, introduced into yeast strain Y187 containing the LacZ gene under the control of the GAL1 promoter, full-length MEKK1 fragments subcloned into the pQE30 expression vector to create pQE-MEKK1 encoding the 6 x His-tagged protein and expressed in Escherichia coli, full-length MEKK1 cDNA fragment with a HA-tag cloned into the pGFPc155c vector encoding the C-terminal part of the GFP protein and transformed to Agrobacterium tumefaciens strain GV3101/pMP90 and injected to Arabidopsis leaves

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
K361M	mutation of the kinase domain of MEKK1 reduces the relative expression of the WRKY53 promoter driven GUS gene from 200% in the presence of intact MEKK1 to 132% in the presence of the mutated form	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Arabidopsis thaliana	5737	-
nucleus	MEKK1/WRKY53 complexes are predominantly localized in the nucleus	Arabidopsis thaliana	5634	-
protoplast	-	Arabidopsis thaliana	-	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	ecotype Columbia	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
epidermal cell	-	Arabidopsis thaliana	-
leaf	MEKK1 binds to the promoter of the WRKY53 gene regulating the switch from a leaf age dependent to a plant age dependent expression	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + WRKY53	phosphorylation increases DNA-binding activity of WRKY53	Arabidopsis thaliana	ADP + phosphorylated WRKY53	-	?

Synonyms

Synonyms	Comment	Organism
MEKK1	-	Arabidopsis thaliana
mitogen activated protein kinase kinase kinase	-	Arabidopsis thaliana