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Literature summary for 2.7.11.25 extracted from
- Activation of MEKK1 by Rho GTPases (2006), Methods Enzymol., 406, 468-478.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | MEKK1 containing residues 1-719 interacts with wild-type RhoA. MEKK1 (149-636) also associates with wild-type RhoA in yeast. The constitutively active mutant of RhoA, RhoAL63, also binds to residues 149-636, whereas the inactive mutant RhoAN19 fails to bind any of the MEKK1 fragments. Interaction between RhoA and MEKK1 seems to be activation-dependent (GTP-binding dependent) in yeast | Mus musculus | |
| additional information | RhoA L63, the active mutant of RhoA, dramatically stimulates the activity of endogenous MEKK1, whereas RhoA wild-type and RhoA N19, a dominant negative mutant of RhoA, show little activation effect | Homo sapiens | |
| additional information | stimulation of MEKK1 kinase activity by RhoA in vitro | synthetic construct |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| MEKK1 cDNA sequence amplified from the pCEP4-HAMEKK1 plasmid and ligated into baculovirus transfer vector pVL1393. Recombinant baculoviruses generated by contransfection with linear wild-type baculoviral DNA into Sf9 cells | synthetic construct |
| MEKK1 fragments (1-132, 149-347, 149-636, 630-772, 766-1173, 766-1493, 1-719, and 565-1174) incorporated into pAS1CYH2 vector, expressed in Saccharomyces cerevisiae Y190 cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C433A | completely abolishes its interaction with RhoA | Mus musculus |
| G452C/R454C/N455D | completely abolishes its interaction with RhoA | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | incubation of MEKK1 with phosphoprotein phosphatases 2A dephosphorylates the protein, significantly reducing the basal activity of MEKK1 generated from insect cells | synthetic construct |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 195000 | - |
SDS-PAGE | synthetic construct |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| synthetic construct | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by centrifugation and on Ni2+-NTA resin | synthetic construct |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK-293 cell | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C | synthetic construct |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MEK4 | - |
synthetic construct | ADP + phosphorylated MEK4 | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MAP kinase kinase kinase | - |
Mus musculus |
| MAP kinase kinase kinase | - |
Homo sapiens |
| MAP kinase kinase kinase | - |
synthetic construct |
| MAP/ERK kinase kinase 1 | - |
Mus musculus |
| MAP/ERK kinase kinase 1 | - |
Homo sapiens |
| MAP/ERK kinase kinase 1 | - |
synthetic construct |
| MAP3K | - |
Mus musculus |
| MAP3K | - |
Homo sapiens |
| MAP3K | - |
synthetic construct |
| MEKK1 | - |
Mus musculus |
| MEKK1 | - |
Homo sapiens |
| MEKK1 | - |
synthetic construct |
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