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Literature summary for 2.7.11.25 extracted from
- Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1 (2007), J. Biol. Chem., 282, 7522-7531.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | phosphorylation of ASK2 at Thr807 increases in response to H2O2 in cells co-expressing ASK2 and a ASK1 kinase-negative mutant, whereas singly expressed ASK2 does not respond to H2O2. ASK2 kinase-negative mutant co-expressed with ASK1 kinase-negative mutant is not phosphorylated at Thr807 irrespective of the H2O2 treatment | Homo sapiens |  |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | ASK2, a highly related serine/threonine kinase to ASK1, functions as a MAP3K only in a heteromeric complex with ASK1 | Homo sapiens |
| additional information | ASK2, a highly related serine/threonine kinase to ASK1, functions as a MAP3K only in a heteromeric complex with ASK1 | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length ASK2 cDNA inserted in pJG4-5, kinase-negative mutant of ASK1 inserted in pEG202. ASK1 and ASK2 constructs co-transformed along with the reporter plasmid pSH18-34 in EGY48 yeast strains, mutants transfected into HEK293 cells and MEF cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | endogenous ASK2 constitutively degraded in ASK1-deficient cells, kinase-negative mutant of ASK1 effectively activates MAP2K and is more competent to respond to oxidative stress than ASK2 alone. Knockdown of ASK2 reveals that ASK2 is required for oxidative stress-induced JNK activation | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| siRNA | transfection of siRNA for ASK1 into cells reduces expression of not only ASK1 but also ASK2 | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O95382 | - |
- |
| Mus musculus | Q9WTR2 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| B-16 cell | - |
Mus musculus | - |
| HEK-293 cell | - |
Homo sapiens | - |
| L-929 cell | - |
Mus musculus | - |
| MEF cell | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + ASK1 | ASK2 activates ASK1 by direct phosphorylation | Homo sapiens | ADP + phosphorylated ASK1 | - |
? | |
| ATP + ASK1 | ASK2 activates ASK1 by direct phosphorylation | Mus musculus | ADP + phosphorylated ASK1 | - |
? | |
| ATP + JNK | activated by ASK2 in complex with ASK1 | Homo sapiens | ADP + phosphorylated JNK | - |
? | |
| ATP + MKK4 | activated by ASK2 in complex with ASK1 | Homo sapiens | ADP + phosphorylated MKK4 | - |
? | |
| ATP + MKK6 | activated by ASK2 in complex with ASK1 | Homo sapiens | ADP + phosphorylated MKK6 | - |
? | |
| ATP + p38 | activated by ASK2 in complex with ASK1 | Homo sapiens | ADP + phosphorylated p38 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| apoptosis signal-regulating kinase 1 | - |
Homo sapiens |
| apoptosis signal-regulating kinase 1 | - |
Mus musculus |
| apoptosis signal-regulating kinase 2 | - |
Homo sapiens |
| apoptosis signal-regulating kinase 2 | - |
Mus musculus |
| ASK1 | - |
Homo sapiens |
| ASK1 | - |
Mus musculus |
| ASK2 | - |
Homo sapiens |
| ASK2 | - |
Mus musculus |
| MAP3K | - |
Homo sapiens |
| MAP3K | - |
Mus musculus |
| MAPKKK6 | - |
Homo sapiens |
| MAPKKK6 | - |
Mus musculus |
| mitogen-activated protein kinase kinase kinase | - |
Homo sapiens |
| mitogen-activated protein kinase kinase kinase | - |
Mus musculus |
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