Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ras | activates C-Raf and B-Raf | Xenopus laevis |
Cloned (Comment) | Organism |
---|---|
B-RAf, DNA and amino acid sequence determination and analysis, expression of His6-tagged B-Raf in bacteria, expression of His6-tagged and FLAG-tagged C-Raf in insect cells | Xenopus laevis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | activation of MEK1 by C-Raf | Xenopus laevis | 5794 | - |
kinetochore | activation of p42 MAPK | Xenopus laevis | 776 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MEK1 | Xenopus laevis | Ras-induced activation of the MAPK signaling cascade | ADP + phosphorylated MEK1 | - |
? | |
ATP + p42 MAPK | Xenopus laevis | Ras-induced activation of the MAPK signaling cascade | ADP + phosphorylated p42 MAPK | - |
? | |
additional information | Xenopus laevis | B-Raf and C-Raf, but not Mos, are required for Ras-induced MEK1 and p42 MAPK activation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of Mos and B-Raf from egg extracts by heparin affinity chromatography and/or ion exchange chromatography, and GST-MEK1 affinity chromatography, recombinant His6-tagged B-Raf by nickel affinity chromatography | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | no changes in B-Raf activity after fertilization | Xenopus laevis | - |
embryo | no changes in B-Raf activity in the first embryonic cell cycles | Xenopus laevis | - |
oocyte | no changes in B-Raf activity during progesterone-induced maturation | Xenopus laevis | - |
zygote | - |
Xenopus laevis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + histone H1 | - |
Xenopus laevis | ADP + phosphorylated histone H1 | - |
? | |
ATP + MEK1 | Ras-induced activation of the MAPK signaling cascade | Xenopus laevis | ADP + phosphorylated MEK1 | - |
? | |
ATP + MEK1 | the substrate of B-Raf and C-Raf is a MAPKK, no substrate of Mos, recombinant GST-MEK1 substrate | Xenopus laevis | ADP + phosphorylated MEK1 | - |
? | |
ATP + p42 MAPK | Ras-induced activation of the MAPK signaling cascade | Xenopus laevis | ADP + phosphorylated p42 MAPK | - |
? | |
ATP + p42 MAPK | the substrate of B-Raf and C-Raf, no substrate of Mos, recombinant His6-p42 MAPK substrate | Xenopus laevis | ADP + phosphorylated p42 MAPK | - |
? | |
additional information | B-Raf and C-Raf, but not Mos, are required for Ras-induced MEK1 and p42 MAPK activation | Xenopus laevis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
B-Raf | - |
Xenopus laevis |
C-Raf | - |
Xenopus laevis |
MAP kinase kinase kinase | - |
Xenopus laevis |
MAPKKK | - |
Xenopus laevis |
Mos | - |
Xenopus laevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Xenopus laevis |