Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.25 extracted from

  • Takekawa, M.; Tatebayashi, K.; Saito, H.
    Conserved docking site is essential for activation of mammalian MAP kinase kinases by specific MAP kinase kinase kinases (2005), Mol. Cell, 18, 295-306.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information a functional DVD docking site of MAPKK substrates is absolutely required for activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of FLAG-tagged MTK1 and wild-type and mutant HA-tagged MKK6 in COS-7 cells, direct and immunoprecipitation study Homo sapiens

Protein Variants

Protein Variants Comment Organism
E1372Q site-directed mutagenesis, the MTK1 mutant shows altered substrate specificity comapred to the wild-type enzyme Homo sapiens
K1371D site-directed mutagenesis, MTK1 mutant catalytic site mutant, inactive mutant, no interaction with substrate MKK6 docking site mutants Homo sapiens
K1371E site-directed mutagenesis, MTK1 mutant catalytic site mutant, inactive mutant, no interaction with substrate MKK6 docking site mutants Homo sapiens
K1371G site-directed mutagenesis, MTK1 mutant catalytic site mutant, inactive mutant, no interaction with substrate MKK6 docking site mutants Homo sapiens
K1371R site-directed mutagenesis, MTK1 mutant catalytic site mutant, inactive mutant, no interaction with substrate MKK6 docking site mutants Homo sapiens
Q1254E site-directed mutagenesis, the MEKK1 mutant shows altered substrate specificity comapred to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information MAPKK with mutated DVD docking sites are inhibitory for the MAPKKK, as well as synthetic DVD sequence oligopeptides of e.g. MKK6, in vitro and in vivo, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + MKK6 Homo sapiens substrate of stress-activated MAPKKKs ASK1 and MTK1 ADP + phosphorylated MKK6
-
?
additional information Homo sapiens the MAPKKKs are part of the MAPK signaling cascade, several structurally diverse MAPKKK families exist, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate structure-function relationship of MAPKKKs and MAPKKs, K1371 is the catalytic residue of MTK1, MTK1, TAK1, and MEKK1 also possess lysine as catalytic residues Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + MKK3
-
Homo sapiens ADP + phosphorylated MKK3
-
?
ATP + MKK4 substrate of MEKK1 mutant Q1254E and of MTK1 mutant E1372Q Homo sapiens ADP + phosphorylated MKK4
-
?
ATP + MKK6 substrate of stress-activated MAPKKKs ASK1 and MTK1 Homo sapiens ADP + phosphorylated MKK6
-
?
ATP + MKK6 phosphorylation in the activation loop, substrate of stress-activated MAPKKKs ASK1 and MTK1, no activity with MKK6 mutants E318P, F327D, I331D, and V328G, but reduced activity with MKK6 mutants V324G and V328A Homo sapiens ADP + phosphorylated MKK6
-
?
additional information the MAPKKKs are part of the MAPK signaling cascade, several structurally diverse MAPKKK families exist, overview Homo sapiens ?
-
?
additional information substrate specificities of MAPKKKs with wild-type and mutant MAPKKs, overview, no activity with MKK7 by MEKK1 mutant Q1254E, wild-type MTK1, and MTK1 mutant E1372Q, the enzyme docks at the DVD docking site of MAPKK, a stretch of about 20 amino acids immediately on the C-terminal side of the catalytic domain, MAPKK with mutated DVD docking sites are inhibitory for the MAPKKK, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
ASK1
-
Homo sapiens
MAP kinase kinase kinase
-
Homo sapiens
MAPKKK
-
Homo sapiens
MEKK1
-
Homo sapiens
MEKK2
-
Homo sapiens
MEKK3
-
Homo sapiens
MEKK4
-
Homo sapiens
MLK
-
Homo sapiens
Mos
-
Homo sapiens
MTK1
-
Homo sapiens
Raf
-
Homo sapiens
TAK1
-
Homo sapiens
TAO
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens