Any feedback?
Literature summary for 2.7.11.25 extracted from
- Subdomain VIII is a specificity-determining region in MEKK1 (2003), J. Biol. Chem., 278, 48498-48505.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged MEKK1 subdomain VIII, comprising residues 1174-1493, His6-tagged wild-type, full-length enzyme, and His6-tagged mutant enzymes F1443A, I1394/L1402A, Q1405R/Q1406R, and L1402A/F1443A in HeLa and COS-1 cells | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F1443A | site-directed mutagenesis, subdomain X mutation, the mutant shows reduced activity compared to the wild-type enzyme | Mus musculus |
| I1394/L1402A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Mus musculus |
| L1402A/F1443A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Mus musculus |
| Q1405R/Q1406R | site-directed mutagenesis C-terminal to the subdomain VIII, creation of an optimal recognition site for protease furin, the mutant binds MKK4 more tightly than the wild-type enzyme, MKK4 binding protects the mutant enzyme from proteolytic cleavage | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MKK4 | Mus musculus | MKK4 activates the JNK signaling pathway | ADP + phosphorylated MKK4 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MKK4 | MKK4 activates the JNK signaling pathway | Mus musculus | ADP + phosphorylated MKK4 | - |
? | |
| ATP + MKK4 | a MAPKK, activity of wild-type and mutant MEKK1 with wild-type and mutant MKK4, determination of the MKK4 binding site of MEKK1, overview | Mus musculus | ADP + phosphorylated MKK4 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | subdomain VIII is a specificity-determining region in MEKK1 | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MEKK1 | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
