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Literature summary for 2.7.11.25 extracted from

  • Calipel, A.; Lefevre, G.; Pouponnot, C.; Mouriaux, F.; Eychene, A.; Mascarelli, F.
    Mutation of B-Raf in human choroidal melanoma cells mediates cell proliferation and transformation through the MEK/ERK Pathway (2003), J. Biol. Chem., 278, 42409-42418.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the mutant enzyme V599E in COS cells leading to 10.7fold increased activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the BRAF gene is mutated in several cancers, especially in cutaneous melanoma Homo sapiens
V599E naturally occurring mutation of cancer cells, the mutation leads to 10fold increased enzyme activity compared to the wild-type enzyme, and constitutive, Ras-independent activation, siRNA-mediated depletion of the mutant enzyme diminishes the enzyme activity and also cell proliferation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
farnesylthiosalicylic acid a Ras antagonist, inhibits the wild-type and V599E mutant enzyme in vivo and in vitro Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information ectopic expression of B-Raf in cutaneous melanoma cells Homo sapiens
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the natural enzyme mutant V599E induces cell transformation in NIH3T3 cells, the B-Raf/MEK/ERK pathway regulates cell cycle proteins, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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 gene BRAF
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Source Tissue

Source Tissue Comment Organism Textmining
melanocyte
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 Homo sapiens
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MKT-BR cell choroidal cancer cell line, expresses V599E mutant enzyme Homo sapiens
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OCM-1 cell choroidal cancer cell line, expresses V599E mutant enzyme Homo sapiens
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SP-6.5 cell choroidal cancer cell line, expresses V599E mutant enzyme Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the natural enzyme mutant V599E induces cell transformation in NIH3T3 cells, the B-Raf/MEK/ERK pathway regulates cell cycle proteins, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
B-Raf
-
 Homo sapiens
B-Raf kinase
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 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens