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Literature summary for 2.7.11.24 extracted from

  • Kohoutova, L.; Kourova, H.; Nagy, S.K.; Volc, J.; Halada, P.; Meszaros, T.; Meskiene, I.; Boegre, L.; Binarova, P.
    The Arabidopsis mitogen-activated protein kinase 6 is associated with gamma-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress (2015), New Phytol., 207, 1061-1074.
    View publication on PubMed
Search for more literature for 2.7.11.24

Activating Compound

Activating Compound Comment Organism Structure
additional information MPK6 is activated by MKK4 MAPK kinase Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged MPK6 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
U0126 the activity of MAPKs is inhibited by treatment of cells with the selective MEK inhibitor Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
microtubule mitotic microtubules Arabidopsis thaliana 5874
-
microtubule mitotic microtubules, MPK6 is recruited to gamma-tubulin or gamma-tubulin complexes Arabidopsis thaliana 5874
-
additional information co-localization of MPK6 and p-ERK with gamma-tubulin and with microtubule plus end protein EB1c in Arabidopsis cells. gamma-Tubulin and ERK partly colocalize on the spindle and prominent close to kinetochores. p-ERK follows the dynamic localization of gamma-tubulin in the anaphase-to-telophase transition in Arabidopsis cells. In anaphase, phosphorylated ERK and EB1c-GFP co-localize on shortening kinetochore fibres Arabidopsis thaliana
-
-
additional information co-localization of MPK6 and phosphorylated ERK with gamma-tubulin and with microtubule plus end protein EB1c in Arabidopsis cells Arabidopsis thaliana
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nucleus
-
 Arabidopsis thaliana 5634
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + EB1c Arabidopsis thaliana the microtubule plus end protein ADP + phosphorylated EB1c
-
 ?
additional information Arabidopsis thaliana MPK6 interacts with gamma-tubulin and co-sediments with plant microtubules polymerized in vitro. The active form of MAP kinase is enriched with microtubules and follows similar dynamics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotypes Landsberg erecta (Ler) and Columbia (Col)
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Arabidopsis thaliana Q39026 ecotypes Landsberg erecta (Ler) and Columbia (Col)
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein MPK6 is activated by MKK4 MAPK kinase Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged MPK6 by metal affinity chromatography, the His-tag is cleaved off by TEV protease Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cell culture ERK is associated with gamma-tubulin on microtubules in proliferating cells Arabidopsis thaliana
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cell culture MPK6 is associated with gamma-tubulin on microtubules in proliferating cells Arabidopsis thaliana
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root meristematic cell
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + EB1c the microtubule plus end protein Arabidopsis thaliana ADP + phosphorylated EB1c
-
 ?
ATP + EB1c the microtubule plus end protein, recombinant GST-tagged substrate protein, phosphorylation on a threonine residue Arabidopsis thaliana ADP + phosphorylated EB1c
-
 ?
ATP + MAP65-1 microtubule-associated protein, phosphorylation in vitro by MPK6, recombinant GST-tagged substrate protein Arabidopsis thaliana ADP + phosphorylated MAP65-1
-
 ?
additional information MPK6 interacts with gamma-tubulin and co-sediments with plant microtubules polymerized in vitro. The active form of MAP kinase is enriched with microtubules and follows similar dynamics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition Arabidopsis thaliana ?
-
 ?
additional information no activity with recombinant GST-tagged EB1a protein. MPK6 is recruited to gamma-tubulin or gamma-tubulin complexes, but no direct phosphorylation of either gamma-tubulin or gamma-tubulin complex protein GCP4 wby MPK6 is detectable in vitro Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
ERK
-
 Arabidopsis thaliana
MAPK6
-
 Arabidopsis thaliana
mitogen-activated protein kinase
-
 Arabidopsis thaliana
mitogen-activated protein kinase 6
-
 Arabidopsis thaliana
MPK6
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 room temperature Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction the mpk6-2 mutant is sensitive to 3-nitro-L-tyrosine (NO2-Tyr) treatment with respect to mitotic abnormalities, and root cells overexpressing the MAP kinase-inactivating phosphatase AP2C3 show defects in chromosome segregation and spindle orientation Arabidopsis thaliana
physiological function the active form of MAP kinase interacts with gamma-tubulin on specific subsets of mitotic microtubules during late mitosis. MPK6 phosphorylates EB1c, but not EB1a, and has a role in maintaining regular planes of cell division under stress conditions. MPK6 activtes the micotubule-associated protein MAP65-1 that has a redundant function in Arabidopsis with MAP65-3 in cytokinesis. MPK6 is required for regulation of the alignment of cell division on NO2-Tyr treatment Arabidopsis thaliana