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Literature summary for 2.7.11.24 extracted from

  • Ding, H.; Zhang, A.; Wang, J.; Lu, R.; Zhang, H.; Zhang, J.; Jiang, M.
    Identity of an ABA-activated 46 kDa mitogen-activated protein kinase from Zea mays leaves: partial purification, identification and characterization (2009), Planta, 230, 239-251.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
abscisic acid ABA Zea mays

Inhibitors

Inhibitors Comment Organism Structure
PD98059
-
Zea mays
U0126
-
Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information apparent Km for myelin basic protein is 0.13 microg microl-1 Zea mays
0.062
-
ATP apparent KM Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM MgCl2 Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
determined by SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a protein Zea mays
-
ADP + a phosphoprotein
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified from maize by Q-Sepharose FF, Phenyl-Sepharose FF, Resource Q, Mono QTM 5/50 GL, poly-L-lysine agarose, and Superdex 75 prep-grade columns Zea mays

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000002
-
homogenate Zea mays
0.000017
-
purification step (NH4)2SO4 30% Zea mays
0.00002
-
purification step centrifugation 100000 X g Zea mays
0.000085
-
purification step Q-Sepharose FF Zea mays
0.00024
-
purification step Phenyl-Sepharose FF Zea mays
0.000834
-
purification step Resource Q Zea mays
0.001804
-
purification step Mono Q 5/50 GL Zea mays
0.009005
-
purification step poly-L-lysine-agarose Zea mays
0.02042
-
purification step HiLoad 16/60 Superdex 75 pg Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a protein
-
Zea mays ADP + a phosphoprotein
-
?
ATP + myelin basic protein
-
Zea mays ADP + phosphorylated myelin basic protein
-
?

Subunits

Subunits Comment Organism
monomer
-
Zea mays

Synonyms

Synonyms Comment Organism
MAP kinase
-
Zea mays
mitogen-activated kinase
-
Zea mays
mitogen-activated protein kinase
-
Zea mays
p46MAPK
-
Zea mays
ZmMAPK5
-
Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
in-gel kinase activity assay at room temperature Zea mays
30
-
-
Zea mays

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
70
-
Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
in-gel kinase activity assay Zea mays
8
-
-
Zea mays

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
ATP
-
Zea mays