Activating Compound | Comment | Organism | Structure |
---|---|---|---|
abscisic acid | ABA | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PD98059 | - |
Zea mays | |
U0126 | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | apparent Km for myelin basic protein is 0.13 microg microl-1 | Zea mays | |
0.062 | - |
ATP | apparent KM | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM MgCl2 | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
determined by SDS-PAGE | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | Zea mays | - |
ADP + a phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially purified from maize by Q-Sepharose FF, Phenyl-Sepharose FF, Resource Q, Mono QTM 5/50 GL, poly-L-lysine agarose, and Superdex 75 prep-grade columns | Zea mays |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000002 | - |
homogenate | Zea mays |
0.000017 | - |
purification step (NH4)2SO4 30% | Zea mays |
0.00002 | - |
purification step centrifugation 100000 X g | Zea mays |
0.000085 | - |
purification step Q-Sepharose FF | Zea mays |
0.00024 | - |
purification step Phenyl-Sepharose FF | Zea mays |
0.000834 | - |
purification step Resource Q | Zea mays |
0.001804 | - |
purification step Mono Q 5/50 GL | Zea mays |
0.009005 | - |
purification step poly-L-lysine-agarose | Zea mays |
0.02042 | - |
purification step HiLoad 16/60 Superdex 75 pg | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
Zea mays | ADP + a phosphoprotein | - |
? | |
ATP + myelin basic protein | - |
Zea mays | ADP + phosphorylated myelin basic protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Zea mays |
Synonyms | Comment | Organism |
---|---|---|
MAP kinase | - |
Zea mays |
mitogen-activated kinase | - |
Zea mays |
mitogen-activated protein kinase | - |
Zea mays |
p46MAPK | - |
Zea mays |
ZmMAPK5 | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
in-gel kinase activity assay at room temperature | Zea mays |
30 | - |
- |
Zea mays |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
70 | - |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
in-gel kinase activity assay | Zea mays |
8 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Zea mays |