Literature summary for 2.7.11.24 extracted from

Wilsbacher, J.L.; Juang, Y.C.; Khokhlatchev, A.V.; Gallagher, E.; Binns, D.; Goldsmith, E.J.; Cobb, M.H.
Characterization of mitogen-activated protein kinase (MAPK) dimers (2006), Biochemistry, 45, 13175-13182.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	increased ability of phosphorylated ERK2 to dimerize as the salt concentration is increased, ERK2 fails to dimerize in the presence of EDTA and EGTA	Homo sapiens

Application

Application	Comment	Organism
additional information	phosphorylated ERK2 has an increased capacity to form dimers relative to unphosphorylated ERK2, dimerization of SAPKalphaI/JNK2 displays no dependence on phosphorylation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
mutated fragments of ERK2 subcloned into wild-type or mutated forms of the bacterial coexpression system plasmid, R4FMEK1 plus ERK2pETHis6. SAPKalphaI/JNK2 expressed from pRSETSAPKalphaI and from the bacterial coexpression system	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E339A	in 100 mM KCl completely dimeric	Homo sapiens
F329A	retains ability to dimerize significantly in 100 mM KCl	Homo sapiens
H176A/F181A/L4A	retains ability to dimerize significantly in 100 mM KCl	Homo sapiens
H176E	retains ability to dimerize significantly in 100 mM KCl	Homo sapiens
H176E/E343A	retains ability to dimerize significantly in 100 mM KCl	Homo sapiens
H176E/L2E/E343A	retains ability to dimerize significantly in 100 mM KCl	Homo sapiens
H176E/L4A	in 100 mM KCl almost completely monomeric	Homo sapiens
K340A	in 100 mM KCl completely dimeric	Homo sapiens
K340E/L3E	retains ability to dimerize significantly in 100 mM KCl	Homo sapiens
L2E	in 100 mM KCl completely dimeric	Homo sapiens
L4A	retains ability to dimerize significantly in 100 mM KCl	Homo sapiens
additional information	at least five residues must be mutated simultaneously to produce an ERK2 mutant that is predominantly monomeric. Mutants, whether monomers or dimers, have specific protein kinase activities under fixed assay conditions that are roughly equivalent to wild-type ERK2	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	partially restores the ability of the protein to dimerize	Homo sapiens
Mg2+	partially restores the ability of the protein to dimerize	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Subunits

Subunits	Comment	Organism
dimer	phosphorylated ERK2 using gel filtration, SAPKRI/JNK2 using sedimentation analysis	Homo sapiens

Synonyms

Synonyms	Comment	Organism
c-Jun N-terminal kinase	-	Homo sapiens
ERK2	-	Homo sapiens
MAPK	-	Homo sapiens
mitogen-activated protein kinase	-	Homo sapiens
SAPKalphaI/JNK2	-	Homo sapiens

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Release 2023.1 (January 2023)