Cloned (Comment) | Organism |
---|---|
His6-tagged ERK1 and p38alpha kinase expressed in Escherichia coli strain BL21 (DE3) | synthetic construct |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
STE7ERK | ERK1 | synthetic construct | |
0.0037 | - |
MEK1ERK | ERK1 | synthetic construct | |
0.0044 | - |
ELKERK | ERK1 | synthetic construct | |
0.0056 | - |
MEK2ERK | ERK1 | synthetic construct | |
0.03 | - |
MEK2ERK | p38alpha kinase | synthetic construct | |
0.065 | - |
MEK1ERK | p38alpha kinase | synthetic construct | |
0.096 | - |
SCRAMMMEK2 | ERK1 | synthetic construct | |
0.127 | - |
ERKSub | ERK1 | synthetic construct | |
0.173 | - |
ERKSTE7 | ERK1 | synthetic construct | |
0.344 | - |
ERKMEK1 | ERK1 | synthetic construct | |
0.388 | - |
ERKMEK2 | ERK1 | synthetic construct | |
1.2 | - |
ERKSub | p38alpha kinase | synthetic construct |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
synthetic construct |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
synthetic construct | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ERK1 and p38alpha kinase purified by NiNTA column | synthetic construct |
Storage Stability | Organism |
---|---|
-70°C, 12.5 mM HEPES-buffer, pH 7.3, 100 mM KCl, 6.25% (v/v) glycerol, 0.5 mM DTT | synthetic construct |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ELKERK | ERK1 | synthetic construct | ? | - |
? | |
ATP + ERKMEK1 | ERK1 | synthetic construct | ? | - |
? | |
ATP + ERKMEK2 | ERK1 | synthetic construct | ? | - |
? | |
ATP + ERKSTE7 | ERK1 | synthetic construct | ? | - |
? | |
ATP + ERKSub | ERK1 and p38alpha kinase | synthetic construct | ? | - |
? | |
ATP + MEK1ERK | ERK1 and p38alpha kinase | synthetic construct | ? | - |
? | |
ATP + MEK2ERK | ERK1 and p38alpha kinase | synthetic construct | ? | - |
? | |
ATP + SCRAMMMEK2 | ERK1 | synthetic construct | ? | - |
? | |
ATP + STE7ERK | ERK1 | synthetic construct | ? | - |
? | |
additional information | a peptide docking sequence derived from either a downstream substrate or an upstream activator is appended to an ERK substrate peptide to yield a high-efficiency substrate for ERK without loss of specificity | synthetic construct | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ERK1 | - |
synthetic construct |
p38alpha kinase | - |
synthetic construct |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
MEK2ERK | p38alpha kinase | synthetic construct | |
2 | - |
MEK1ERK | - |
synthetic construct | |
2.167 | - |
SCRAMMMEK2 | - |
synthetic construct | |
2.3 | - |
MEK2ERK | - |
synthetic construct | |
2.3 | - |
ELKERK | - |
synthetic construct | |
2.67 | - |
ERKSTE7 | - |
synthetic construct | |
2.83 | - |
ERKMEK1 | - |
synthetic construct | |
2.83 | - |
STE7ERK | - |
synthetic construct | |
2.94 | - |
SCRAMMMEK2 | - |
synthetic construct | |
3 | - |
ERKMEK2 | - |
synthetic construct | |
3.13 | - |
ERKSub | - |
synthetic construct | |
4.167 | - |
ERKSub | - |
synthetic construct | |
9.5 | - |
ERKSub | p38alpha kinase | synthetic construct | |
9.5 | - |
MEK1ERK | p38alpha kinase | synthetic construct | |
16.67 | - |
MEK2ERK | p38alpha kinase | synthetic construct |