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Literature summary for 2.7.11.24 extracted from
- Identification and characterization of ERK MAP kinase phosphorylation sites in Smad3 (2005), Biochemistry, 44, 12546-12553.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EGF | induces phosphorylation of Smad3 | Mus musculus | |
| additional information | ERK2 is activated by phosphorylation | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | PD98059 and U0126 inhibit EGF-induced phosphorylation of Smad3 | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Smad3 | Mus musculus | substrate of MAPKs, e.g. ERK2 | ADP + phosphorylated Smad3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | ERK2 is activated by phosphorylation | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| C2C12 cell | - |
Mus musculus | - |
| myoblast | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Elk1 | recombinant GST-tagged Elk1, substrate of ERK2 | Mus musculus | ADP + phosphorylated Elk1 | - |
? | |
| ATP + myelin basic protein | substrate of ERK2 | Mus musculus | ADP + phosphorylated myelin basic protein | - |
? | |
| ATP + Smad3 | substrate of MAPKs, e.g. ERK2 | Mus musculus | ADP + phosphorylated Smad3 | - |
? | |
| ATP + Smad3 | substrate of MAPKs, e.g. ERK2, identification of phosphorylation sites Ser203, Ser207, and Thr187, Ser207 is the best phosphorylation site for ERK2, other MAPKs than ERK2 also phosphorylate Ser212 | Mus musculus | ADP + phosphorylated Smad3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ERK | - |
Mus musculus |
| ERK2 | - |
Mus musculus |
| MAP kinase | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
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