Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the recombinant detagged enzyme is activated by specific phosphorylation at Thr180 and Tyr182 through recombinant GST-tagged MKK6 mutant S207E/T211E | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged p38 isozyme alpha in Escherichia coli strain BL21(DE3) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
protein ATF2 | pH 7.6, 27°C, purified, recombinant detagged, activated p38 MAPKalpha | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | as MgATP2- | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the p38 MAPKalpha is involved in cell signal transduction and mediates responses to cell stresses and to growth factors | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | p38 MAPKalpha is activated by phosphorylation at Thr180 and Tyr182 through recombinant GST-tagged MKK6 mutant S207E/T211E, for activation and specific dual phosphorylation of the recombinant enzyme, the His-tag needs to be removed by thrombin, because it can cause unspecific phosphorylation at other sites | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged p38 isozyme alpha from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography to over 98% purity, removal of the His-tag by thrombin is required for propper enzyme purification and activation | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein ATF2 | recombinant GST-tagged ATF2DELTA115 | Homo sapiens | ADP + phosphorylated protein ATF2 | - |
? | |
additional information | the p38 MAPKalpha is involved in cell signal transduction and mediates responses to cell stresses and to growth factors | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43472-43556, unphosphorylated to trifold phosphorylated p38 MAPKalpha, mass spectrometry | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
p38 MAPKalpha | - |
Homo sapiens |
p38 mitogen-activated protein kinase alpha | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
protein ATF2 | pH 7.6, 27°C, purified, recombinant detagged, activated p38 MAPKalpha | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | as MgATP2- | Homo sapiens |