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Literature summary for 2.7.11.24 extracted from

  • Zhu, H.; Klemic, J.F.; Chang, S.; Bertone, P.; Casamayor, A.; Klemic, K.G.; Smith, D.; Gerstein, M.; Reed, M.A.; Snyder, M.
    Analysis of yeast protein kinases using protein chips (2000), Nat. Genet., 26, 283-289.
    View publication on PubMed
Search for more literature for 2.7.11.24

Application

Application Comment Organism
analysis development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic tree of kinases derived from the kinase core sequence, overview, overexpression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a protein Saccharomyces cerevisiae
-
 ADP + a phosphoprotein
-
 ?
additional information Saccharomyces cerevisiae Hog1 is related to osmotic stress ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein Hog1 performs autophosphorylation, while Fus3 does not Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a protein
-
 Saccharomyces cerevisiae ADP + a phosphoprotein
-
 ?
ATP + Axl2 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Axl2
-
 ?
ATP + casein substrate of Hog1 Saccharomyces cerevisiae ADP + phosphocasein
-
 ?
ATP + cdc42 substrate of Gic2 Saccharomyces cerevisiae ADP + phosphorylated cdc42
-
 ?
ATP + Gic2 substrate of Fus3, and of Hog1 Saccharomyces cerevisiae ADP + phosphorylated Gic2
-
 ?
ATP + histone H1 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-histone H1
-
 ?
ATP + Hog1D substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Hog1D
-
 ?
ATP + Hsl1 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Hsl1
-
 ?
ATP + MBP substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-MBP
-
 ?
ATP + Mek1 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Mek1
-
 ?
ATP + protein tyrosine kinase 2 substrate of Hog1 Saccharomyces cerevisiae ADP + phosphorylated protein tyrosine kinase 2
-
 ?
ATP + RAD9 high activity with Fus3, low activity with Hog1 Saccharomyces cerevisiae ADP + phospho-RAD9
-
 ?
ATP + RAD9p substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-RAD9p
-
 ?
ATP + Red1 preferred substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Red1
-
 ?
ATP + Swe1 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Swe1
-
 ?
ATP + Swi6 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Swi6
-
 ?
ATP + Tub4p substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Tub4
-
 ?
additional information Hog1 is related to osmotic stress Saccharomyces cerevisiae ?
-
 ?
additional information the enzyme performs autophosphorylation Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Fus3
-
 Saccharomyces cerevisiae
Hog1
-
 Saccharomyces cerevisiae
Mek1p
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae