Any feedback?
Literature summary for 2.7.11.23 extracted from
- The RNA polymerase II CTD kinase Ctk1 functions in translation elongation (2007), Genes Dev., 21, 1409-1421.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | functions in translation by enhancing decoding fidelity, Ctk1 interacts with the transcription and mRNA export complex, which couples transcription to mRNA export, may bind to correctly processed mRNPs during transcription and accompany the mRNP to the ribosomes in the cytoplasm, where Ctk1 enhances efficient and accurate translation of the mRNA, function of Ctk1 in translation seems to be conserved in evolution | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | depletion of Ctk1, causes a defect in translation, impairment of growth, hypersensitivity to inhibitors of translation elongation, addition of increasing amounts of CTDK-I complex to the Ctk1-depleted extracts restores the translation elongation defect as well as the defect in correct decoding of the message in a dose-dependent manner | Saccharomyces cerevisiae |
| additional information | Ctk1-depleted as well as substrate rps2 S238A mutant cells show a dfect in translation elongation through an increase in the frequency of miscoding, phenotype, overview | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| anisomycin | inhibits translation elongation of wild-type | Saccharomyces cerevisiae |  |
| cycloheximide | inhibits translation elongation of wild-type | Saccharomyces cerevisiae | |
| geneticin | inhibits translation elongation of wild-type | Saccharomyces cerevisiae | |
| hygromycin B | inhibits translation elongation of wild-type | Saccharomyces cerevisiae | |
| additional information | translation elongation of wild-type not inhibited by paromomycin | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | monosome | Saccharomyces cerevisiae | - |
- |
| nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
| polysome | - |
Saccharomyces cerevisiae | 5844 | - |
| polysome | Ctk1 is associated with polysomes | Saccharomyces cerevisiae | 5844 | - |
| ribosome | is associated with translating ribosomes in vivo | Saccharomyces cerevisiae | 5840 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Rps2 | Saccharomyces cerevisiae | a protein of the small ribosomal subunit, Ctk1 interacts with the transcription and mRNA export, TREX, complex, which couples transcription to mRNA export, and Ctk1 enhances efficient and accurate translation of the mRNA, Ctk1 is a prerequisite for correct decoding in vivo overview | ADP + phosphorylated Rps2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native Ctk1 complex | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Rps2 | phosphorylates on Ser 238, phosphorylation of Rps2 is needed for translational accuracy | Saccharomyces cerevisiae | ? | - |
? | |
| ATP + Rps2 | a protein of the small ribosomal subunit, Ctk1 interacts with the transcription and mRNA export, TREX, complex, which couples transcription to mRNA export, and Ctk1 enhances efficient and accurate translation of the mRNA, Ctk1 is a prerequisite for correct decoding in vivo overview | Saccharomyces cerevisiae | ADP + phosphorylated Rps2 | - |
? | |
| ATP + Rps2 | a protein of the small ribosomal subunit, phosphorylation at Ser238 | Saccharomyces cerevisiae | ADP + phosphorylated Rps2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ctk1 | - |
Saccharomyces cerevisiae |
| RNA polymerase II CTD kinase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
