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Literature summary for 2.7.11.23 extracted from

  • Dvir, A.; Stein, L.Y.; Calore, B.L.; Dynan, W.S.
    Purification and characterization of a template-associated protein kinase that phosphorylates RNA polymerase II (1993), J. Biol. Chem., 268, 10440-10447.
    View publication on PubMed
Search for more literature for 2.7.11.23

Activating Compound

Activating Compound Comment Organism Structure
DNA activation Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 1 * 67000 + 1 * 83000, component B, SDS-PAGE Homo sapiens
83000
-
 1 * 67000 + 1 * 83000, component B, SDS-PAGE Homo sapiens
180000
-
 gel filtration, enzyme component B Homo sapiens
340000
-
 gel filtration, enzyme component A Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] Homo sapiens
-
 ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two components: A and B Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
-70°C, glycerol, 20% v/v Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + CTD-containing fusion proteins e.g. GAL4-CTD (formerly GC147) or HSP 90 Homo sapiens ADP + ?
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
-
 Homo sapiens ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates not Tyr-residues Homo sapiens ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] phosphorylates Ser- and Thr-residues equally Homo sapiens ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
 ?
GTP + [DNA-directed eukaryotic RNA polymerase II subunit IIa] poor substrate Homo sapiens GDP + ?
-
 ?
additional information human enzyme consists of 2 components: component A bears the active site and is capable of DNA-independent autophosphorylation, component B stimulates component A and is phosphorylated only in the presence of DNA Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 67000 + 1 * 83000, component B, SDS-PAGE Homo sapiens