Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyclin D1 | CDK4 is dependent on | Mus musculus | |
cyclin D1 | CDK4 is dependent on | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type cyclin D1/CDK4 in HeLa cells, coexpression of cyclin D1 or cyclin D1T286A along with either CDK4 or CDK4(K35M) in HeLa cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K35m | site-directed mutagenesis of CDK4 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MEP50 protein | Mus musculus | a PRMT5 co-regulatory factor | ADP + MEP50 phosphoprotein | - |
? | |
ATP + MEP50 protein | Homo sapiens | a PRMT5 co-regulatory factor | ADP + MEP50 phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | and other cancer cell lines | Homo sapiens | - |
lymphoma cell | primary tumors of a mouse lymphoma model | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MEP50 protein | a PRMT5 co-regulatory factor | Mus musculus | ADP + MEP50 phosphoprotein | - |
? | |
ATP + MEP50 protein | a PRMT5 co-regulatory factor | Homo sapiens | ADP + MEP50 phosphoprotein | - |
? | |
ATP + MEP50 protein | a PRMT5 co-regulatory factor, purified recombinant PRMT5/MEP50 produced in Sf9 cells or HeLa cells, phosphorylation of Thr5 and perhaps Ser264 by CDK4 | Homo sapiens | ADP + MEP50 phosphoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDK4 kinase | - |
Mus musculus |
CDK4 kinase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus | |
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the primary cellular mechanism that restricts cyclin D1/CDK4 activity is cytoplasmic, ubiquitin-mediated degradation of cyclin D1 during S-phase. Mutations that disrupt this event, via within the cyclin D1 degron or inactivating the cyclin D1 E3 ligase, Fbx4, directly contribute to neoplastic growth | Mus musculus |
metabolism | the primary cellular mechanism that restricts cyclin D1/CDK4 activity is cytoplasmic, ubiquitin-mediated degradation of cyclin D1 during S-phase. Mutations that disrupt this event, via within the cyclin D1 degron or inactivating the cyclin D1 E3 ligase, Fbx4, directly contribute to neoplastic growth | Homo sapiens |
physiological function | nuclear cyclin D1/CDK4-dependent repression of CUL4A and CUL4B, encoding scaffolding proteins for the E3 ligase that directs CDT1 degradation during S-phase, the repression requires S-phase accumulation of catalytically active cyclin D1/CDK4, molecular mechanism, overview. Cyclin D1/CDK4 can increase histone methyltransferase activity of PRMT5/MEP50, a PRMT5 co-regulatory factor, through phosphorylation of Thr5 and perhaps through Ser264, and increased PRMT5 activity mediates key events associated with cyclin D1-dependent neoplastic growth including CUL4 repression, CDT1 overexpression, and DNA re-replication. Cyclin D1T286A/CDK4 regulates PRMT5 methyltransferase activity via MEP50 phosphorylation, overview | Homo sapiens |
physiological function | nuclear cyclin D1/CDK4-dependent repression of CUL4A and CUL4B, encoding scaffolding proteins for the E3 ligase that directs CDT1 degradation during S-phase, the repression requires S-phase accumulation of catalytically active cyclin D1/CDK4, molecular mechanism, overview. Phosphorylation of MEP50, a PRMT5 co-regulatory factor, by CDK4 increases PRMT5/MEP50 activity, and increased PRMT5 activity mediates key events associated with cyclin D1-dependent neoplastic growth including CUL4 repression, CDT1 overexpression, and DNA re-replication | Mus musculus |