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Literature summary for 2.7.11.22 extracted from

  • Harashima, H.; Shinmyo, A.; Sekine, M.
    Phosphorylation of threonine 161 in plant cyclin-dependent kinase A is required for cell division by activation of its associated kinase (2007), Plant J., 52, 435-448.
    View publication on PubMed
Search for more literature for 2.7.11.22

Activating Compound

Activating Compound Comment Organism Structure
cyclin
-
 Nicotiana tabacum
additional information phosphorylation of T161 in CDKA.1 is essential for cell division during male gametophyte development Arabidopsis thaliana
additional information phosphorylation of T161 is required for CDKA activation Nicotiana tabacum
additional information phosphorylation of T161 in plant CDKA is required for activation of its associated kinase Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the dominant-negative CDKA mutant D146N or the double mutant D146N/T161A fused with GFP in BY-2 cells, Escherichia coli expressing GST-fused CDKA and CDKB Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
D146N overexpression results in elongated cells after cell division is arrested, shows phosphorylation of T161 Nicotiana tabacum
D146N mutant forms kinase-inactive complexes Nicotiana tabacum
D146N/T161A retains a dominant-negative effect on cellular growth, shows phosphorylation of T161 Nicotiana tabacum
K33R the ATP-binding site is inactivated, no kinase activity Nicotiana tabacum
additional information loss-of-function mutation in the CDKA.1 gene, displays male gametophyte lethality, and produces bicellular pollen grains instead of the tricellular grains produced in wild-type plants Arabidopsis thaliana
T161A non-phosphorylatable mutant, overexpression has no effect on cellular growth, can bind to cyclins, but its relative binding affinity differs between CYCD2.1 and CYCD3.3 when compared to the wild-type, recombinant CDKA-T161A/cyclin D complex possesses no detectable kinase activity Nicotiana tabacum
T161A mutant forms kinase-inactive complexes Nicotiana tabacum
T161E shows no phosphorylation of T161 Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P24100 ecotype Columbia Col-0
-
Nicotiana tabacum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Arabidopsis thaliana
-
TBY-2 cell
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone H1
-
 Nicotiana tabacum ADP + phosphorylated histone H1
-
 ?
additional information phosphorylation of T161 in plant CDKA is required for activation of its associated kinase Nicotiana tabacum ?
-
 ?

Synonyms

Synonyms Comment Organism
A-type cyclin-dependent kinase A
-
 Nicotiana tabacum
A-type cyclin-dependent kinase A
-
 Arabidopsis thaliana
CDKA
-
 Nicotiana tabacum
CDKA
-
 Arabidopsis thaliana
CDKB
-
 Nicotiana tabacum
cyclin-dependent kinase A
-
 Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
cyclin D
-
 Nicotiana tabacum