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Literature summary for 2.7.11.22 extracted from

  • Wohlbold, L.; Larochelle, S.; Liao, J.C.; Livshits, G.; Singer, J.; Shokat, K.M.; Fisher, R.P.
    The cyclin-dependent kinase (CDK) family member PNQALRE/CCRK supports cell proliferation but has no intrinsic CDK-activating kinase (CAK) activity (2006), Cell Cycle, 5, 546-554.
    View publication on PubMed
Search for more literature for 2.7.11.22

Activating Compound

Activating Compound Comment Organism Structure
additional information cyclin H or cyclin H together with cyclin K do not activate PNQALRE Mus musculus

Application

Application Comment Organism
additional information no support of the proposed function of PNQALRE/CCRK in activating CDKs, but instead reinforcement of the notion that Cdk7 is the major, and to date the only, CAK in mammalian cells Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
full-length PNQALRE cDNA (splice variant 1) expressed in HEK 293, U2OS and HCT116 cells, PNQALRE and components of the CAK trimer coexpressed in insect Sf9 cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
RNAi depletion of PNQALRE by more than 80%, impairs cell proliferation, but fails to arrest the cell cycle at a discrete point Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 immunoblot analysis from testis Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9JHU3
-
-

Purification (Commentary)

Purification (Comment) Organism
by metal-ion affinity and subsequent gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain low expression Mus musculus
-
heart low expression Mus musculus
-
kidney low expression Mus musculus
-
liver low expression Mus musculus
-
lung low expression Mus musculus
-
skeletal muscle low expression Mus musculus
-
skin low expression Mus musculus
-
small intestine low expression Mus musculus
-
spleen low expression Mus musculus
-
stomach low expression Mus musculus
-
testis highest expression Mus musculus
-
thymus high expression Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no detectable kinase activity towards CDKs, the Pol II carboxyl-terminal domain (CTD), histone H1 or myelin basic protein Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
CCRK
-
 Mus musculus
CDK
-
 Mus musculus
cyclin-dependent kinase
-
 Mus musculus
p42
-
 Mus musculus
PNQALRE
-
 Mus musculus